ISOLATION OF NEW NONSENSE AND FRAMESHIFT MUTANTS IN THE IMMUNOGLOBULIN-MU HEAVY-CHAIN GENE OF HYBRIDOMA CELLS

被引：19

作者：

CONNOR, A ^{[1
]}

COLLINS, C ^{[1
]}

JIANG, L ^{[1
]}

MCMASTER, M ^{[1
]}

SHULMAN, MJ ^{[1
]}

机构：

[1] UNIV TORONTO, DEPT MED GENET, TORONTO M5S 1A8, ONTARIO, CANADA

来源：

SOMATIC CELL AND MOLECULAR GENETICS | 1993年 / 19卷 / 04期

关键词：

D O I：

10.1007/BF01232744

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to expand the experimental material available for genetic and biochemical analyses of the natural immunoglobulin genes, we have isolated a variety of mutant mouse hybridoma cell lines. Some of these mutants have partial or complete deletions of the mu gene. Other mutants have nonsense or frameshift mutations in the exons encoding the variable and the second and third constant region domains of the mu heavy chain. When combined with earlier mutant data, this collection of genotypically and phenotypically tight mutants of known sequence spans most of the 10 kb of the A gene, providing material for a variety of studies of genetic recombination and mRNA metabolism.

引用

页码：313 / 320

页数：8

共 41 条

[1] TIMING, GENETIC REQUIREMENTS AND FUNCTIONAL CONSEQUENCES OF SOMATIC HYPERMUTATION DURING B-CELL DEVELOPMENT [J].

ALLEN, D ;

CUMANO, A ;

DILDROP, R ;

KOCKS, C ;

RAJEWSKY, K ;

RAJEWSKY, N ;

ROES, J ;

SABLITZKY, F ;

SIEKEVITZ, M .

IMMUNOLOGICAL REVIEWS, 1987, 96 :5-22

[2] HOMOLOGOUS RECOMBINATION CAN RESTORE NORMAL IMMUNOGLOBULIN PRODUCTION IN A MUTANT HYBRIDOMA CELL-LINE [J].

BAKER, MD ;

PENNELL, N ;

BOSNOYAN, L ;

SHULMAN, MJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6432-6436

[3] NONSENSE MUTATIONS IN THE HUMAN BETA-GLOBIN GENE AFFECT MESSENGER-RNA METABOLISM [J].

BASERGA, SJ ;

BENZ, EJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (07) :2056-2060

[4] BETA-GLOBIN NONSENSE MUTATION - DEFICIENT ACCUMULATION OF MESSENGER-RNA OCCURS DESPITE NORMAL CYTOPLASMIC STABILITY [J].

BASERGA, SJ ;

BENZ, EJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :2935-2939

[5] CONSEQUENCES OF FRAMESHIFT MUTATIONS AT THE IMMUNOGLOBULIN HEAVY-CHAIN LOCUS OF THE MOUSE [J].

BAUMANN, B ;

POTASH, MJ ;

KOHLER, G .

EMBO JOURNAL, 1985, 4 (02) :351-359

[6] PREMATURE TRANSLATION TERMINATION MEDIATES TRIOSEPHOSPHATE ISOMERASE MESSENGER-RNA DEGRADATION [J].

DAAR, IO ;

MAQUAT, LE .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (02) :802-813

[7]

DAVIS AC, 1989, J IMMUNOL, V143, P1352

[8] ON THE STRUCTURE OF POLYMERIC IGM [J].

DAVIS, AC ;

ROUX, KH ;

SHULMAN, MJ .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (07) :1001-1008

[9] MULTIPLE DNA-SEQUENCE ELEMENTS ARE NECESSARY FOR THE FUNCTION OF AN IMMUNOGLOBULIN HEAVY-CHAIN PROMOTER [J].

EATON, S ;

CALAME, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7634-7638

[10] BASE SUBSTITUTIONS, FRAMESHIFTS, AND SMALL DELETIONS CONSTITUTE IONIZING RADIATION-INDUCED POINT MUTATIONS IN MAMMALIAN-CELLS [J].

GROSOVSKY, AJ ;

DEBOER, JG ;

DEJONG, PJ ;

DROBETSKY, EA ;

GLICKMAN, BW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (01) :185-188

← 1 2 3 4 5 →