A SYNTAXIN-RELATED PROTEIN CONTROLS ACETYLCHOLINE-RELEASE BY DIFFERENT MECHANISMS IN APLYSIA

被引:18
作者
SMIRNOVA, T
FOSSIER, P
STINNAKRE, J
MALLET, J
BAUX, G
机构
[1] CNRS,GENET MOLEC NEUROTRANSMISS & PROC NEURODEGENERAT,F-91198 GIF SUR YVETTE,FRANCE
[2] CNRS,NEUROBIOL CELLULAIRE & MOLEC LAB,F-91198 GIF SUR YVETTE,FRANCE
关键词
D O I
10.1016/0306-4522(95)00134-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Polyclonal antibodies raised against rat syntaxin-1B and an affinity-purified fraction have been used to study the functional role of this protein in transmitter release from Aplysia neurons. In a ganglionic protein extract, this fraction recognized a 37,000 molecular weight protein which therefore might be the Aplysia homologue of rat brain syntaxin-1B. Immunoglobulins were injected in the presynaptic cell of an identified cholinergic synapse of the buccal ganglion of Aplysia californica. This treatment decreased the postsynaptic response due to a reduction of the number of quanta released in relation to a decline of presynaptic Ca2+ current. When antibodies were applied extracellularly, transmitter release also decreased. In contrast to intracellular injection, this reduction was not accompanied by a decrease of the Ca2+ current but by an increase of presynaptic outward current. When injected in the presynaptic neuron, syntaxin complementary RNA also depressed Ca2+ current and transmission. This work provides evidence that Aplysia neurons express a syntaxin-like protein which is involved in the control of the presynaptic Ca2+ influx triggering acetylcholine release from terminals. This protein appears to have an extracellular segment which might interact with outward current.
引用
收藏
页码:125 / 133
页数:9
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