IMMOBILIZATION OF MICROORGANISMS BY ADHESION - INTERPLAY OF ELECTROSTATIC AND NONELECTROSTATIC INTERACTIONS

被引:187
作者
MOZES, N [1 ]
MARCHAL, F [1 ]
HERMESSE, MP [1 ]
VANHAECHT, JL [1 ]
REULIAUX, L [1 ]
LEONARD, AJ [1 ]
ROUXHET, PG [1 ]
机构
[1] CATHOLIC UNIV LOUVAIN,UNITE CHIM INTERFACES,PL CROIX SUD 1,B-1348 LOUVAIN LA NEUVE,BELGIUM
关键词
BIOCHEMICAL ENGINEERING - BIOLOGICAL MATERIALS - Cells - ENZYMES - Immobilization;
D O I
10.1002/bit.260300315
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The adhesion of three microorganisms (Saccharomyces cerevisiae, Acetobacter aceti, and Moniliella pollinis) to different materials has been studied using various supports (glass, metals, plastics), some of which were treated by an Fe(III) solution. Saccharomyces cerevisiae and A. aceti adhere to metals under certain pH conditions but do not adhere to any of the other materials tested unless it is previously treated by ferric ions; adhesion of these hydrophilic cells is essentially controlled by electrostatic interactions. Moniliella pollinis adhere spontaneously to glass and to polymeric materials, but its attachment is also influenced by cell-cell or cell-support electrostatic repulsions; near the cell isoelectric point cell flocculation is competing with adhesion to a support.
引用
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页码:439 / 450
页数:12
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