TRANSCRIPTION OF THE TRPR GENE OF ESCHERICHIA-COLI - AN AUTOGENEOUSLY REGULATED SYSTEM STUDIED BY DIRECT MEASUREMENTS OF MESSENGER-RNA LEVELS INVIVO

被引：13

作者：

BOGOSIAN, G

SOMERVILLE, RL

NISHI, K

KANO, Y

IMAMOTO, F

机构：

[1] PURDUE UNIV, DEPT BIOCHEM, W LAFAYETTE, IN 47907 USA

[2] INST PHYS & CHEM RES, WAKO, SAITAMA 351, JAPAN

来源：

MOLECULAR AND GENERAL GENETICS | 1984年 / 193卷 / 02期

关键词：

D O I：

10.1007/BF00330675

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of the trpR gene of E. coli was investigated by measuring trpR mRNA levels in vivo under various physiological conditions. Trp repressor, when present, led to significant decreases in the amount of trpR message produced; this effect was enhanced by providing excess L-tryptophan to the system. In the absence of Trp repressor, no changes in trpR message levels were observed under any of the conditions employed. Sedimentation profiles of trpR mRNA revealed a single species under all circumstances. Apparently, autogenous repression alone acts to regulate transcription of the trpR gene. The activity of the trpR promoter in vivo was evaluated using a trpR-lacZ operon fusion. Very good agreement was found between relative promoter activity and trpR levels under all experimental conditions.

引用

页码：244 / 250

页数：7

共 35 条

[1] NUCLEOTIDE-SEQUENCE OF PROMOTER-OPERATOR REGION OF TRYPTOPHAN OPERON OF ESCHERICHIA-COLI [J].