LINEAR CONTOUR LENGTH DEPENDENCE OF ELECTRICAL POLARIZABILITY OF NUCLEOSOMAL DNA FRAGMENTS - IMPLICATIONS FOR THE FLEXIBILITY OF DNA

被引：2

作者：

HACQUES, MF

MARION, C

机构：

[1] Laboratoire de Physico-Chimie Biologique, LBTM-CNRS UM 24, Bat. 731 Université Claude Bernard de Lyon, 69622 Villeurbanne Cédex, 43 Bd. du

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90985-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transient electric birefringence of monodisperse ollgonucleosomal DNA ranging from 145 to 990 base pairs has been studied. The orientation of fragments can be described in terms of an induced dipole moment with a small contribution of a permanent dipole. The electrical polarizability Δα was found to increase linearly with the DNA contour length. This unexpected dependence might result from a bent structure of DNA already considerable for very short segments. The observed Δα values agree with a segmental orientation of rigld subunits of length 13-18 nm as estimated in the elastic model of DNA with a kink angle of about 41°. © 1990.

引用

页码：1140 / 1145

页数：6

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