PURIFICATION AND POLYPEPTIDE COMPOSITION OF CORPORA-AMYLACEA FROM AGED HUMAN-BRAIN

被引：20

作者：

STEYAERT, A ^{[1
]}

CISSE, S ^{[1
]}

MERHI, Y ^{[1
]}

KALBAKJI, A ^{[1
]}

REID, N ^{[1
]}

GAUVREAU, D ^{[1
]}

LACOSTEROYAL, G ^{[1
]}

机构：

[1] UNIV QUEBEC,INRS SANTE,245 BLVD HYMUS,POINTE CLAIRE H9R 1G6,QUEBEC,CANADA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1990年 / 31卷 / 01期

关键词：

Ageing; Brain protein; Corpora amylacea; Polyglucosan body; Purification;

D O I：

10.1016/0165-0270(90)90010-D

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Corpora amylacea (CA) accumulation in the brain is a normal correlate of ageing. The presence of a small amount of protein in these polyglucosan bodies is a consistent finding, although the nature of this protein material remains unknown. Using sucrose gradient fractionation and density centrifugation on Percoll, a method was developed to obtain highly pure preparations of CA from human brain. The protein content of isolated CA was estimated to be approx. 4% of the total fraction by weight. SDS-PAGE analysis of CA fractions showed several polypeptide bands with molecular weights ranging from 24 to 133 kDa. Four of these bands with molecular weights of 133, 94, 42 and 24 kDa are more abundant. Thus, pure preparations of CA can be obtained that are suitable for protein analysis. © 1990.

引用

页码：59 / 64

页数：6

共 17 条

[1] ON THE NATURE, ORIGIN AND DISTRIBUTION OF THE CORPORA AMYLACEA OF THE BRAIN WITH OBSERVATIONS ON SOME NEW STAINING REACTIONS [J].

ALDER, N .

JOURNAL OF MENTAL SCIENCE, 1953, 99 (417) :689-697

[2] INTRANEURITIC CORPORA AMYLACEA - DEMONSTRATION IN ORBITAL CORTEX OF ELDERLY SUBJECTS BY MEANS OF EARLY POSTMORTEM BRAIN SAMPLING AND ELECTRON-MICROSCOPY [J].

ANZIL, AP ;

HERRLINGER, H ;

BLINZINGER, K ;

KRONSKI, D .

VIRCHOWS ARCHIV ABTEILUNG A PATHOLOGISCHE ANATOMIE, 1974, 364 (04) :297-301

[3] MORPHOLOGICAL AND CHEMICAL CHARACTERIZATION OF A STARCH GRANULE-LIKE POLYGLUCOSAN DEPOSIT ISOLATED FROM HUMAN-BRAIN [J].

BOBIN, SA ;

WISNIEWSKI, HM ;

KIERAS, FJ ;

IQBAL, K .

ACTA NEUROPATHOLOGICA, 1981, 55 (01) :47-52

[4] Norms for some structural changes in the human cerebellum from birth to old age [J].

Ellis, RS .

JOURNAL OF COMPARATIVE NEUROLOGY, 1920, 32 (01) :1-34

[5]

FLEMING P D, 1986, Society for Neuroscience Abstracts, V12, P1314

[6]

FLEMING P D, 1987, Neurology, V37, P157

[7] MYOCLONIC EPILEPSY WITH LAFORA BODIES - SOME ULTRASTRUCTURAL, HISTOCHEMICAL, AND BIOCHEMICAL ASPECTS [J].

GAMBETTI, P ;

DIMAURO, S ;

HIRT, L ;

BLUME, RP .

ARCHIVES OF NEUROLOGY, 1971, 25 (06) :483-&

[8] PROGRESSIVE MYOCLONUS EPILEPSY WITH LAFORA INCLUSION BODIES .I. CLINICAL GENETIC HISTOPATHOLOGIC AND BIOCHEMICAL ASPECTS [J].

JANEWAY, R ;

RAVENS, JR ;

PEARCE, LA ;

ODOR, DL ;

SUZUKI, K .

ARCHIVES OF NEUROLOGY, 1967, 16 (06) :565-+

[9] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

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