EVALUATION OF A NONRADIOACTIVE DNA PROBE FOR DETECTING PORPHYROMONAS-GINGIVALIS IN SUBGINGIVAL SPECIMENS

被引：29

作者：

TAY, F ^{[1
]}

LIU, YB ^{[1
]}

FLYNN, MJ ^{[1
]}

SLOTS, J ^{[1
]}

机构：

[1] UNIV SO CALIF,SCH DENT,DEPT PERIODONTOL,MC 0641,LOS ANGELES,CA 90089

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1992年 / 7卷 / 06期

关键词：

PORPHYROMONAS-GINGIVALIS; DNA PROBE; SUBGINGIVAL PLAQUE; TRANSPORT MEDIUM; COLONY BLOT; ANAEROBIC CULTURE; MICROBIAL DIAGNOSTICS;

D O I：

10.1111/j.1399-302X.1992.tb00634.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study compared the ability of a nonradioactive digoxigenin-labeled DNA probe and anaerobic culture to identify subgingival Porphyromonas gingivalis. Total cellular DNA from P gingivalis ATCC 33277T was labeled using the Genius(TM) kit from Boehringer Mannheim Biochemicals. Anaerobic culture was performed using VMGA III transport medium and enriched brucella blood agar. The DNA probe could detect as little as 1000 P gingivalis cells added to supragingival plaque. Also, the probe could detect P gingivalis when it was present in proportions too low to be visualized on overgrown bacterial plates. The probe showed no visible reaction with strains of various oral species or with thousands of non-P gingivalis colonies from plaque samples. VMGA III could maintain the viability of P gingivalis for up to 6 days, as evidenced by DNA probing of colony blot of subgingival cultures. A total cellular DNA probe for detecting P gingivalis seems to offer a simple and reliable method of detecting the organism in subgingival specimens.

引用

页码：344 / 348

页数：5

共 14 条

[1]

Coykendall AL, Kaczmarek FS, Slots J., Int J Syst Bacteriol, 30, pp. 559-564

[2]

Loesche WJ, Lopatin DE, Stoll J, van Poperin N, Hujoel PP, Comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard, J Clin Microbiol, 30, pp. 418-426, (1992)

[3]

Moller AJ., Microbiological examination of root canals and periapical tissues of human teeth, Odontol Tidskr, 74, pp. 1-380, (1966)

[4]

Murray PA, French CK, DNA probe detection of periodontal pathogens, New biotechnology in oral research, pp. 33-53, (1989)

[5]

Slots J., Importance of black‐pigmented Bacteroides in human periodontal disease, Host‐parasite interactions in periodontal diseases, pp. 27-45, (1982)

[6]

Slots J., Detection of colonies of Bacteroides gingivalis by a rapid fluorescence assay for trypsin‐like activity, Oral Microbiol Immunol, 2, pp. 139-141, (1987)

[7]

Slots J, Reynolds HS, Long‐wave UV‐light fluorescence for identification of black‐pigmented Bacteroides spp, J Clin Microbiol, 16, pp. 1148-1151, (1982)

[8]

Slots J, Listgarten MA, Bacteroides gingivalis, Bacteroides intermedius and Actinobacillus actinomycetemcomitans in human periodontal diseases, J Clin Periodontol, 15, pp. 85-93, (1988)

[9]

Slots J, Rams TE, Methods for the study of oral microorganisms, Contemporary oral microbiology and immunology, pp. 275-282, (1992)

[10]

Slots J, Rams TE, Microbiology of periodontal disease, Contemporary oral microbiology and immunology, pp. 425-443, (1992)

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