CARBOHYDRATE PROTEIN INTERACTIONS BETWEEN THE CUTICLE OF INFECTIVE JUVENILES OF MELOIDOGYNE-INCOGNITA AND SPORES OF THE OBLIGATE HYPERPARASITE PASTEURIA-PENETRANS

被引:33
作者
DAVIES, KG
DANKS, C
机构
[1] AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire
[2] AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Hertfordshire
来源
NEMATOLOGICA | 1993年 / 39卷 / 01期
关键词
D O I
10.1163/187529293X00033
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
Standard assays were done to test the effects of various preincubation treatments on the attachment of spores of Pasteuria penetrans to the cuticles of second-stage juveniles (J2) of Meloidogyne incognita. Either J2s or spores were preincubated in a series of proteolytic, lipolytic and glycolytic enzymes, carbohydrates, detergents and solubilising agents and attachment assays performed. J2 and spores were also incubated in a series of fluorescent labelled lectins and examined using a fluorescence microscope. The surface of the J2 appears to contain carbohydrate recognition domains which are probably not collagen and these interact with N-acetylglucosamine moeities on the spore surface which are linked to either glycoproteins or peptidoglycans. As exposing the spores to lysozyme (muramidase) weakens attachment the spores also contain N-acetylmuraminic acid.
引用
收藏
页码:53 / 64
页数:12
相关论文
共 35 条
[1]  
Bird A.F., 1989, PARASITOLOGY, V15, P301, DOI 10. 1017/S0031182000059801
[2]   STUDIES ON THE SURFACE-COAT (GLYCOCALYX) OF THE DAUER LARVA OF ANGUINA-AGROSTIS [J].
BIRD, AF ;
ZUCKERMAN, BM .
INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1989, 19 (03) :235-240
[3]   STUDIES ON THE PROPERTIES OF THE SPORES OF SOME POPULATIONS OF PASTEURIA-PENETRANS [J].
BIRD, AF ;
BRISBANE, PG ;
MCCLURE, SG ;
KIMBER, RWL .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1990, 55 (02) :169-178
[4]   INCREASED CROP YIELDS FOLLOWING APPLICATION OF BACILLUS-PENETRANS TO FIELD PLOTS INFESTED WITH MELOIDOGYNE-INCOGNITA [J].
BROWN, SM ;
KEPNER, JL ;
SMART, GC .
SOIL BIOLOGY & BIOCHEMISTRY, 1985, 17 (04) :483-486
[5]   PROTEINS INVOLVED IN THE ATTACHMENT OF A HYPERPARASITE, PASTEURIA-PENETRANS, TO ITS PLANT-PARASITIC NEMATODE HOST, MELOIDOGYNE-INCOGNITA [J].
DAVIES, KG ;
ROBINSON, MP ;
LAIRD, V .
JOURNAL OF INVERTEBRATE PATHOLOGY, 1992, 59 (01) :18-23
[6]   MICROBIAL AGENTS FOR THE BIOLOGICAL-CONTROL OF PLANT-PARASITIC NEMATODES IN TROPICAL AGRICULTURE [J].
DAVIES, KG ;
DELEIJ, FAAM ;
KERRY, BR .
TROPICAL PEST MANAGEMENT, 1991, 37 (04) :303-320
[7]  
DAVIS EL, 1988, J NEMATOL, V20, P609
[8]  
FINNE J, 1982, METHOD ENZYMOL, V83, P269
[9]  
FUJIMOTO D, 1973, ARCH BIOCHEM BIOPHYS, V103, P1
[10]  
HOOPER DJ, 1986, LABORATORY METHODS W