DISSIMILATION OF AROMATIC-COMPOUNDS IN RHODOTORULA-GRAMINIS - BIOCHEMICAL-CHARACTERIZATION OF PLEIOTROPICALLY NEGATIVE MUTANTS

被引:38
作者
DURHAM, DR [1 ]
MCNAMEE, CG [1 ]
STEWART, DB [1 ]
机构
[1] GENEX CORP, DEPT MICROBIAL BIOCHEM, GAITHERSBURG, MD 20877 USA
关键词
D O I
10.1128/JB.160.2.771-777.1984
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Microorganisms oxidize many aromatic compounds through the dihydroxylated intermediates catechol and protocatechuate and through the .beta.-ketoadipate pathway. The catabolic sequences used by the yeast R. graminis for the dissimilation of aromatic compounds were elucidated after biochemical analysis of pleiotropically negative mutant strains. Growth properties of 1 mutant strain revealed that benzoate-4 hydroxylase was required for the utilization of phenylalanine, mandelate and benzoate. Analysis of benzoate 4-hydroxylase- and p-hydroxybenzoate hydroxylase-deficient mutants provided genetic evidence that benzoate was hydroxylated in the para position forming p-hydroxybenzoate. Enzyme assays and growth studies with wild-type and mutant strains of R. graminis indicated that separate and highly specific hydroxylases oxidized p-hydroxybenzoate and m-hydroxybenzoate to protocatechuate. Examination of a protocatechuate 3,4-dioxygenase-deficient mutant demonstrated the role of the protocatechuate branch of the eucaryotic .beta.-ketoadipate pathway for the utilization of phenylalanine, mandelate, benzoate and m-hydroxybenzoate. Salicylate was metabolized through catechol. R. graminis differs from other yeasts such as Trichosporon cutaneum and R. mucilaginosa in that it contains both branches of the .beta.-ketodipate pathway.
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页码:771 / 777
页数:7
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