ELECTROSTATIC ASSAY OF PHOSPHOLIPASE-A ACTIVITY - AN APPLICATION OF THE 2ND HARMONIC METHOD OF MONITORING MEMBRANE BOUNDARY POTENTIALS

被引：5

作者：

MIRSKY, VM

CHERNY, VV

SOKOLOV, VS

MARKIN, VS

机构：

[1] Frumkin Institute of Electrochemistry, the U.S.S.R. Academy of Sciences, Moscow

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 21卷 / 04期

关键词：

Bilayer lipid membrane; Boundary; Enzyme assay; Phospholipase A; Surface potential;

D O I：

10.1016/0165-022X(90)90002-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To evaluate phospholipase A activity a new assay is suggested. This assay is based on the recording of boundary potential of the planar bilayer during enzymatic hydrolysis of lipids. To register these changes, a second harmonic method is used. Sensitivity of the assay is about 0.0002 units/ml regardless of the impurities that may be present in the samples. On eanalysis takes about 5 min. © 1990.

引用

页码：277 / 284

页数：8

共 19 条

[1] VOLTAGE-DEPENDENT CAPACITANCE IN LIPID BILAYERS MADE FROM MONOLAYERS [J].