DISTRIBUTION OF A NEGATIVE REGULATOR OF HEMATOPOIETIC STEM-CELL PROLIFERATION (ACSDKP) AND THYMOSIN-BETA-4 IN MOUSE-TISSUES

被引：66

作者：

PRADELLES, P ^{[1
]}

FROBERT, Y ^{[1
]}

CREMINON, C ^{[1
]}

IVONINE, H ^{[1
]}

FRINDEL, E ^{[1
]}

机构：

[1] INSERM, FAC MED & PHARM, BIOCHIM MED LAB, F-87025 LIMOGES, FRANCE

来源：

FEBS LETTERS | 1991年 / 289卷 / 02期

关键词：

NEGATIVE HEMATOPOIETIC REGULATOR; ACSDKP TETRAPEPTIDE; THYMOSIN-BETA-4; ENZYME IMMUNOASSAY; ACETYLCHOLINESTERASE; MOUSE TISSUE;

D O I：

10.1016/0014-5793(91)81062-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A competitive enzyme immunoassay using acetylcholinesterase as tracer for thymosin beta-4, has been developed. Using this assay and a previously described EIA for AcSDKP, a negative regulator of pluripotent haematopoietic stem cell proliferation, the levels of these two peptides were determined in mouse tissue extracts. The combination of EIAs with different HPLC procedures validated these methods and clearly demonstrated the ubiquity of these peptides in mouse tissues. Similar results are reported for rabbit thymus which suggest different hypotheses for AcSDKP biosynthesis.

引用

页码：171 / 175

页数：5

共 29 条

[1] DISTRIBUTION OF THYMOSIN-BETA-4 IN VERTEBRATE CLASSES [J].

ERICKSONVIITANEN, S ;

RUGGIERI, S ;

NATALINI, P ;

HORECKER, BL .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1983, 221 (02) :570-576

[2]

FRINDEL E, 1977, EXP HEMATOL, V5, P74

[3]

FRINDEL E, 1989, LEUKEMIA, V3, P753

[4]

GOMEZMARQUEZ J, 1989, J IMMUNOL, V143, P2740

[5]

GONDO H, 1987, J IMMUNOL, V139, P3840

[6]

GOODALL GJ, 1983, J IMMUNOL, V131, P821

[7] INVOLVEMENT OF THYMOSIN-BETA-4 AND ENDOPROTEINASE ASP-N IN THE BIOSYNTHESIS OF THE TETRAPEPTIDE ACSERASPLYSPRO A REGULATOR OF THE HEMATOPOIETIC SYSTEM [J].

GRILLON, C ;

RIEGER, K ;

BAKALA, J ;

SCHOTT, D ;

MORGAT, JL ;

HANNAPPEL, E ;

VOELTER, W ;

LENFANT, M .

FEBS LETTERS, 1990, 274 (1-2) :30-34

[8]

GUIGON M, 1982, CANCER RES, V42, P638

[9]

GUIGON M, 1990, EXP HEMATOL, V18, P1112

[10] ONE-STEP PROCEDURE FOR THE DETERMINATION OF THYMOSIN-BETA-4 IN SMALL TISSUE SAMPLES AND ITS SEPARATION FROM OTHER THYMOSIN-BETA-4-LIKE PEPTIDES BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].

HANNAPPEL, E .

ANALYTICAL BIOCHEMISTRY, 1986, 156 (02) :390-396

← 1 2 3 →