A REGION OF THE CORONAVIRUS INFECTIOUS-BRONCHITIS VIRUS 1A POLYPROTEIN ENCODING THE 3C-LIKE PROTEASE DOMAIN IS SUBJECT TO RAPID TURNOVER WHEN EXPRESSED IN RABBIT RETICULOCYTE LYSATE

被引:13
作者
TIBBLES, KW
BRIERLEY, I
CAVANAGH, D
BROWN, TDK
机构
[1] UNIV CAMBRIDGE,DEPT PATHOL,DIV VIROL,CAMBRIDGE CB2 1QP,ENGLAND
[2] COMPTON LAB,INST ANIM HLTH,NEWBURY RG20 7NN,BERKS,ENGLAND
基金
英国医学研究理事会;
关键词
D O I
10.1099/0022-1317-76-12-3059
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In order to investigate the mechanisms involved in the processing of infectious bronchitis virus polyproteins, several candidate regions of the genome have been cloned and expressed in vitro. During these studies it was observed that the translation product encoded by one of these clones (pKT205) was poorly expressed. Biochemical and genetic analyses revealed that the basis for the poor expression was a post-translational event involving ubiquitination of the protein and degradation by an ATP-dependent system operating in the reticulocyte lysate used for the in vitro expression. Two independently acting regions which conferred instability were identified, one of which mapped to the predicted 3C protease domain, contained within the 5' end of the clone, while the other, more C-terminal region, was effective in conferring instability upon a heterologous protein to which it had been transferred. These regions may influence the stability of the authentic viral protein(s) in vivo and hence allow for the control of their expression and/or function at the level of proteolysis by cellular protease(s).
引用
收藏
页码:3059 / 3070
页数:12
相关论文
共 46 条
  • [1] INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE
    BACHMAIR, A
    FINLEY, D
    VARSHAVSKY, A
    [J]. SCIENCE, 1986, 234 (4773) : 179 - 186
  • [2] IDENTIFICATION OF A DOMAIN REQUIRED FOR AUTOPROTEOLYTIC CLEAVAGE OF MURINE CORONAVIRUS GENE-A POLYPROTEIN
    BAKER, SC
    SHIEH, CK
    SOE, LH
    CHANG, MF
    VANNIER, DM
    LAI, MMC
    [J]. JOURNAL OF VIROLOGY, 1989, 63 (09) : 3693 - 3699
  • [3] IDENTIFICATION OF THE CATALYTIC SITES OF A PAPAIN-LIKE CYSTEINE PROTEINASE OF MURINE CORONAVIRUS
    BAKER, SC
    YOKOMORI, K
    DONG, S
    CARLISLE, R
    GORBALENYA, AE
    KOONIN, EV
    LAI, MMC
    [J]. JOURNAL OF VIROLOGY, 1993, 67 (10) : 6056 - 6063
  • [4] COMPLETION OF THE SEQUENCE OF THE GENOME OF THE CORONAVIRUS AVIAN INFECTIOUS-BRONCHITIS VIRUS
    BOURSNELL, MEG
    BROWN, TDK
    FOULDS, IJ
    GREEN, PF
    TOMLEY, FM
    BINNS, MM
    [J]. JOURNAL OF GENERAL VIROLOGY, 1987, 68 : 57 - 77
  • [5] AN EFFICIENT RIBOSOMAL FRAME-SHIFTING SIGNAL IN THE POLYMERASE-ENCODING REGION OF THE CORONAVIRUS IBV
    BRIERLEY, I
    BOURSNELL, MEG
    BINNS, MM
    BILIMORIA, B
    BLOK, VC
    BROWN, TDK
    INGLIS, SC
    [J]. EMBO JOURNAL, 1987, 6 (12) : 3779 - 3785
  • [6] CHARACTERIZATION OF AN EFFICIENT CORONAVIRUS RIBOSOMAL FRAMESHIFTING SIGNAL - REQUIREMENT FOR AN RNA PSEUDOKNOT
    BRIERLEY, I
    DIGARD, P
    INGLIS, SC
    [J]. CELL, 1989, 57 (04) : 537 - 547
  • [7] BRIERLEY I, 1990, CORONAVIRUSES THEIR, P275
  • [8] Brown T., 1995, CORONAVIRIDAE VIRUSE, P191, DOI [10.1007/978-1-4899-1531-3_10, DOI 10.1007/978-1-4899-1531-3_10]
  • [9] RECOMMENDATIONS OF THE CORONAVIRUS STUDY-GROUP FOR THE NOMENCLATURE OF THE STRUCTURAL PROTEINS, MESSENGER-RNAS, AND GENES OF CORONAVIRUSES
    CAVANAGH, D
    BRIAN, DA
    ENJUANES, L
    HOLMES, KV
    LAI, MMC
    LAUDE, H
    SIDDELL, SG
    SPAAN, W
    TAGUCHI, F
    TALBOT, PJ
    [J]. VIROLOGY, 1990, 176 (01) : 306 - 307
  • [10] MULTIPLE UBIQUITIN-CONJUGATING ENZYMES PARTICIPATE IN THE IN-VIVO DEGRADATION OF THE YEAST MAT-ALPHA-2 REPRESSOR
    CHEN, P
    JOHNSON, P
    SOMMER, T
    JENTSCH, S
    HOCHSTRASSER, M
    [J]. CELL, 1993, 74 (02) : 357 - 369