IMPAIRED CYTOKINE PRODUCTION IN WHOLE-BLOOD CELL-CULTURES FROM PATIENTS WITH COLORECTAL CARCINOMAS AS COMPARED TO BENIGN COLORECTAL TUMORS AND CONTROLS

Cytokine production was investigated in whole blood cell cultures from 74 patients with colorectal carcinomas, 20 patents with benign colorectal tumors, and 314 healthy controls. in the 4 day post induction supernatants the levels of IFN-gamma, IL-1-alpha, IL-2, and TNF-alpha were measured by a sensitive immunoassay. In the blood cell cultures of the patents with colorectal carcinomas significantly lower values of IFN-gamma (P less-than-or-equal-to 001), IL-1-alpha (P less-than-or-equal-to .001), and IL-2 (P less-than-or-equal-to .01) were found as compared to the patients with benign tumors and the controls, although total and differential leukocyte counts were similar in all three groups. A linear correlation between the levels of IFN-gamma and IL-1-alpha and the tumor stages could be shown. Our results suggest that a growing tumor burden may induce increasing immunological deficiencies as reflected by a decreasing cytokine production of the immune cells.