A YAC CONTIG ENCOMPASSING THE XRCC5 (KU80) DNA-REPAIR GENE AND COMPLEMENTATION OF DEFECTIVE CELLS BY YAC PROTOPLAST FUSION

被引：13

作者：

BLUNT, T

TACCIOLI, GE

PRIESTLEY, A

HAFEZPARAST, M

MCMILLAN, T

LIU, J

COLE, CC

WHITE, J

ALT, FW

JACKSON, SP

SCHURR, E

LEHMANN, AR

JEGGO, PA

机构：

[1] UNIV SUSSEX,MRC,CELL MUTAT UNIT,BRIGHTON BN1 9RR,E SUSSEX,ENGLAND

[2] CHILDRENS HOSP,HOWARD HUGHES MED INST,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,DEPT GENET,BOSTON,MA 02115

[4] HARVARD UNIV,SCH MED,CTR BLOOD RES,BOSTON,MA 02115

[5] MCGILL UNIV,MONTREAL GEN HOSP,RES INST,CTR STUDY HOST RESISTANCE,MONTREAL,PQ H3G 1A4,CANADA

[6] SANGER CTR,CAMBRIDGE CB10 1RQ,ENGLAND

[7] ADDENBROOKES HOSP,DEPT MED,CAMBRIDGE CB2 2QQ,ENGLAND

[8] WELLCOME CRC INST,CAMBRIDGE CB2 1QR,ENGLAND

来源：

GENOMICS | 1995年 / 30卷 / 02期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/geno.1995.9871

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Chinese hamster ovary xrs mutants are sensitive to ionizing radiation, defective in DNA double-strand break rejoining, and unable to carry out V(D)J recombination effectively. Recently, the gene defective in these mutants, XRCC5, has been shown to encode Ku80, a component of the Ku protein and DNA-dependent protein kinase. We present here a YAC contig involving 25 YACs mapping to the region 2q33-q34, which encompasses the XRCC5 gene. Eight new markers for this region of chromosome 2 are identified. YACs encoding the Ku80 gene were transferred to xrs cells by protoplast fusion, and complementation of all the defective phenotypes has been obtained with two YACs. We discuss the advantages and disadvantages of this approach as a strategy for cloning human genes complementing defective rodent cell lines. (C) 1995 Academic Press, Inc.

引用

页码：320 / 328

页数：9

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