THE MITOGENIC PRINCIPLE OF ESCHERICHIA-COLI LIPOPROTEIN - B-LYMPHOCYTE MITOGENICITY OF THE SYNTHETIC ANALOG PALMITOYL-TETRAPEPTIDE (PAM-SER-SER-ASN-ALA)

被引：15

作者：

BESSLER, WG ^{[1
]}

COX, M ^{[1
]}

WIESMULLER, KH ^{[1
]}

JUNG, G ^{[1
]}

机构：

[1] UNIV TUBINGEN, INST ORGAN CHEM, D-7400 TUBINGEN 1, FED REP GER

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1984年 / 121卷 / 01期

关键词：

D O I：

10.1016/0006-291X(84)90687-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Palmitoyl-(S)-seryl-(S)-seryl-(S)-asparaginyl-(S)-alanine (palmitoyl-tetrapeptide) is an analog of the N-terminal part of the lipoprotein from the outer membrane of E. coli. It was prepared by chemical synthesis and tested for biological activity in in vitro lymphocyte culture systems. In spleen cells of the inbred mouse strains C3H/HeJ, C3H/He/Bom/nunu, and Balb/c, the compound exhibited stimulatory activity towards B-lymphocytes comparable to the effect of native lipoprotein, as measured by the incorporation of 3H-thymidine and 3H-uridine, and by a hemolytic plaque assay. The B-lymphocyte tumor cell line BCL 1 was also activated by the compound. The N-terminal tetrapeptide moiety of lipoprotein, linked to a lipophilic molecule, constitutes by itself a novel B-lymphocyte mitogen.

引用

页码：55 / 61

页数：7

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