A NONTRADITIONAL ROLE FOR WATER IN THE CYTOCHROME-C-OXIDASE REACTION

被引：97

作者：

KORNBLATT, JA ^{[1
]}

HOA, GHB ^{[1
]}

机构：

[1] INST BIOL PHYS CHIM,INSERM,UNITE 310,F-75005 PARIS,FRANCE

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 40期

关键词：

D O I：

10.1021/bi00492a010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The passage of electrons through cytochrome c oxidase is directly related to the activity of water. Reducing the activity in a system containing reductant, oxygen, and cytochrome oxidase blocks electron transfer between reduced cytochrome a and oxidized cytochrome a3. The extent of the block is directly related to the osmotic pressure of the system, implying that the protein shell of the oxidase acts as a semipermeable membrane that excludes osmotic perturbants but not water. It appears that approximately 10 water molecules must enter and leave the oxidase in order for internal electron transfer to occur. © 1990, American Chemical Society. All rights reserved.

引用

页码：9370 / 9376

页数：7

共 35 条

[1] OXYGEN PULSED CYTOCHROME-C OXIDASE - FUNCTIONAL PROPERTIES AND CATALYTIC RELEVANCE
ANTONINI, E
BRUNORI, M
COLOSIMO, A
GREENWOOD, C
WILSON, MT
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) : 3128 - 3132
[2] Balny C, 1989, HIGH PRESSURE RES, V2, P1
[3] ANALYSIS OF THE CU, FE, AND ZN CONTENTS IN CYTOCHROME-C OXIDASES FROM DIFFERENT SPECIES AND TISSUES BY PROTON-INDUCED X-RAY-EMISSION (PIXE)
BOMBELKA, E
RICHTER, FW
STROH, A
KADENBACH, B
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 140 (03) : 1007 - 1014
[4] PURIFICATION OF CYTOCHROME-C OXIDASE RETAINING ITS PULSED FORM
BRANDT, U
SCHAGGER, H
VONJAGOW, G
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (03): : 705 - 711
[5] CYTOCHROME-C-OXIDASE - SUBUNIT STRUCTURE AND PROTON PUMPING
BRUNORI, M
ANTONINI, G
MALATESTA, F
SARTI, P
WILSON, MT
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 169 (01): : 1 - 8
[6] BRUNORI M, 1979, J BIOL CHEM, V254, P769
[7] DIXON M, 1979, ENZYMES, P164
[8] Douzou P., 1977, CRYOBIOCHEMISTRY INT
[9] BOVINE HEART CYTOCHROME-C OXIDASE PREPARATIONS CONTAIN HIGH-AFFINITY BINDING-SITES FOR MAGNESIUM AS WELL AS FOR ZINC, COPPER, AND HEME IRON
EINARSDOTTIR, O
CAUGHEY, WS
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 129 (03) : 840 - 847
[10] CATALYTIC ACTIVITY OF CYTOCHROME-OXIDASE AND CYTOCHROME-C IN APOLAR SOLVENTS CONTAINING PHOSPHOLIPIDS AND LOW AMOUNTS OF WATER
ESCAMILLA, E
AYALA, G
DEGOMEZPUYOU, MT
GOMEZPUYOU, A
MILLAN, L
DARSZON, A
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1989, 272 (02) : 332 - 343

← 1 2 3 4 →