A HUMAN DNA TELOMERIC FRAGMENT CONTAINS YEAST ARS AND MITOTIC STABILIZING SEQUENCES

被引：5

作者：

ASCENZIONI, F

DELLAVALLE, G

GUERRINI, AM

PISANI, G

BIONDI, O

DONINI, P

机构：

[1] UNIV ROME LA SAPIENZA,SEZ SCI MICROBIOL,DIPARTIMENTO BIOL CELLULARE & SVILUPPO,I-00185 ROME,ITALY

[2] UNIV PAVIA,DIPARTIMENTO GENET & MICROBIOL A BUZZATI TRAVERSO,I-27100 PAVIA,ITALY

[3] UNIV CATANIA,DIPARTMENTO BIOL ANIM,I-95124 CATANIA,ITALY

来源：

RESEARCH IN MICROBIOLOGY | 1990年 / 141卷 / 09期

关键词：

DNA; ARS; TELOMERE; CHROMOSOME; INSITU HYBRIDIZATION; MITOTIC STABILITY; YEAST; HUMANS; LIBRARY;

D O I：

10.1016/0923-2508(90)90085-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A minilibrary of human DNA fragments was prepared in the vector YIP5 from a DNA preparation enriched for telomeric sequences. Screening of the library produced one clone that hybridized to the TTAGGG sequence. The cloned DNA fragment was shown to be telomeric by a number of criteria. In situ hybridization to metaphase human chromosomes showed that the fragment hybridized to the tips of all human chromosomes. The fragment contained at least two yeast autonomously replicating sequences (ARS) and stabilizing sequences, since it transformed Saccharomyces cerevisiae with high efficiency, giving rise to clones which were mitotically stable under non-selective growth.

引用

页码：1117 / 1129

页数：13

共 31 条

[1] TELOMERIC REPEAT FROM T-THERMOPHILA CROSS HYBRIDIZES WITH HUMAN TELOMERES [J].