Isolation of LERK-5: A ligand of the eph-related receptor tyrosine kinases

被引：48

作者：

Cerretti, DP ^{[1
]}

Bos, TV ^{[1
]}

Nelson, N ^{[1
]}

Kozlosky, CJ ^{[1
]}

Reddy, P ^{[1
]}

Maraskovsky, E ^{[1
]}

Park, LS ^{[1
]}

Lyman, SD ^{[1
]}

Copeland, NG ^{[1
]}

Gilbert, DJ ^{[1
]}

Jenkins, NA ^{[1
]}

Fletcher, FA ^{[1
]}

机构：

[1] NCI,FREDERICK CANC RES & DEV CTR,ABL BASIC RES PROGRAM,MAMMALIAN GENET LAB,FREDERICK,MD 21702

来源：

MOLECULAR IMMUNOLOGY | 1995年 / 32卷 / 16期

关键词：

ligand; receptor tyrosine kinase; eph-family;

D O I：

10.1016/0161-5890(95)00108-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hek and elk are members of the eph-related family of receptor tyrosine kinases. Recently we isolated four cDNAs encoding membrane-bound ligands to hek and elk [Beckmann et al. (1994) EMBO J. 13, 3757-3762; Kozlosky et al. (1995) Oncogene 10, 299-306]. Because of the promiscuous nature of their binding, we have termed these proteins ligands of the eph-related kinases or LERKs. A search of GenBank revealed an expressed sequence tag (EST) with homology to the LERKs. Using this EST as a probe, we have isolated human and murine cDNAs that encode a protein which we call LERK-5. The human and murine cDNAs encode proteins of 333 and 336 amino acids, respectively, with a 97% amino acid identity; LERK-5 has an amino acid identity of 27-59% with the other reported LERKs. LERK-5 is a ligand for both elk and hek and induces receptor phosphorylation. It is expressed in adult lung and kidney and the fetal tissues heart, lung, kidney, and brain. In addition, Southern blot analysis of DNA from interspecific backcross mice indicated that LERK-5 (Eplg5) maps to the proximal region of mouse chromosome 8.

引用

页码：1197 / 1205

页数：9

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