CONSTITUTIVE EXPRESSION OF HEAT-SHOCK PROTEIN-70 IN MAMMALIAN-CELLS CONFERS THERMORESISTANCE

被引：194

作者：

ANGELIDIS, CE

LAZARIDIS, I

PAGOULATOS, GN

机构：

[1] Laboratory of General Biology, University of Ioannina, Medical School

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 199卷 / 01期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16088.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A 70-kDa heat-shock-protein (hsp70) expression vector which contains the human hsp70 gene linked to the human beta-actin promoter, was constructed and used to transfect CV1 monkey cells. Stably transfected CV1 clones were isolated which constitutively synthesized increased amounts of hsp70 at normal temperature. It is shown that these clones are resistant to elevated temperature. This finding indicates that hsp70 is involved in the protection of the cells against a lethal heat treatment and maybe responsible for the phenomenon of thermotolerance.

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页码：35 / 39

页数：5

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