THE MITOGENIC EFFECT OF PLATELET-DERIVED GROWTH-FACTOR IN HUMAN HEPATIC STELLATE CELLS REQUIRES CALCIUM INFLUX

被引：52

作者：

FAILLI, P ^{[1
]}

RUOCCO, C ^{[1
]}

DEFRANCO, R ^{[1
]}

CALIGIURI, A ^{[1
]}

GENTILINI, A ^{[1
]}

GIOTTI, A ^{[1
]}

GENTILINI, P ^{[1
]}

PINZANI, M ^{[1
]}

机构：

[1] UNIV FLORENCE, IST MED INTERNA, CTR INTERUNIV FISIOPATOL EPAT, I-50134 FLORENCE, ITALY

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1995年 / 269卷 / 05期

关键词：

INTRACELLULAR CALCIUM; IMAGE ANALYSIS; THYMIDINE INCORPORATION; CELL DENSITY; MITOGENESIS; HEPATIC FIBROGENESIS;

D O I：

10.1152/ajpcell.1995.269.5.C1133

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 [细胞生物学]; 090102 [作物遗传育种];

摘要：

Platelet-derived growth factor (PDGF) is a key mitogen for hepatic stellate cells (HSC) and has been shown to be implicated in liver tissue repair and fibrogenesis. In this study the relationship between PDGF-induced intracellular Ca2+ concentration ([Ca2+](i)) increase and mitogenesis in cultured human HSC was evaluated. In high-density cell cultures (80-90% subconfluence), PDGF induced a significant increase in [Ca2+](i), characterized by a short-lasting peak phase, which was followed by a long-lasting plateau phase. The plateau phase was abolished in the absence of extracellular Ca2+. However, in low-density cell cultures (30-40% subconfluence), the plateau phase was absent or markedly less pronounced. In parallel sets of experiments, PDGF was significantly less effective in inducing mitogenesis in low-density cell cultures than in high-density cell cultures and was totally ineffective in the absence of extracellular Ca2+. These results suggest that 1) spatial and time dynamics of PDGF-induced [Ca2+](i) increase are dependent on cell density and 2) PDGF-induced mitogenesis requires extracellular Ca2+ influx.

引用

页码：C1133 / C1139

页数：7

共 21 条

[1]

RECEPTOR TYROSINE KINASES [J].