IN-VITRO EFFECTS OF BRAIN-DERIVED NEUROTROPHIC FACTOR RELEASED FROM MICROSPHERES

被引：42

作者：

MITTAL, S ^{[1
]}

COHEN, A ^{[1
]}

MAYSINGER, D ^{[1
]}

机构：

[1] MONTREAL GEN HOSP,CTR RES NEUROSCI,RES INST,MONTREAL,PQ H3G 1A4,CANADA

来源：

NEUROREPORT | 1994年 / 5卷 / 18期

关键词：

MICROENCAPSULATION; BDNF; CHITOSAN; PLGA; MESENCEPHALIC DOPAMINERGIC NEURONS; RETINAL GANGLIA; DORSAL ROOT GANGLIA; NEUROTROPHIN DELIVERY;

D O I：

10.1097/00001756-199412000-00043

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

THE principal objective of these studies was to microencapsulate brain derived neurotrophic factor (BDNF) and to test its biological effects in vitro. Two types of microspheres with BDNF were prepared using biodegradable polymers: chitosan and poly-(L-lactic acid):poly-(L-glycolic acid). Depending on the procedure, sizes ranged from 1 mu m to 1 mm as determined by an image analysis system. The biological assays confirmed that the released BDNF remains biologically active in vitro. Microencapsulation of BDNF and other trophic factors may represent a viable alternative to currently employed delivery systems in vivo and holds potential for eventual therapy of neurodegenerative disorders, in particular for peripheral neuropathies and CNS disorders where supplementation of neurotrophins retards or even prevents neural degeneration.

引用

页码：2577 / 2582

页数：6

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