TRANSFORMING GROWTH-FACTOR-BETA-2 ENHANCES THE OSTEOINDUCTIVE ACTIVITY OF A BOVINE BONE-DERIVED FRACTION CONTAINING BONE MORPHOGENETIC PROTEIN-2 AND PROTEIN-3

被引：57

作者：

BENTZ, H ^{[1
]}

THOMPSON, AY ^{[1
]}

ARMSTRONG, R ^{[1
]}

CHANG, RJ ^{[1
]}

PIEZ, KA ^{[1
]}

ROSEN, DM ^{[1
]}

机构：

[1] BERLEX,S SAN FRANCISCO,CA 94080

来源：

MATRIX | 1991年 / 11卷 / 04期

关键词：

BONE MORPHOGENETIC PROTEIN; CARTILAGE; OSTEOINDUCTION; TRANSFORMING GROWTH FACTOR-BETA;

D O I：

10.1016/S0934-8832(11)80234-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously identified a novel glycoprotein in an osteoinductive fraction from bovine bone (Bentz et al.: Amino acid sequence of bovine osteoinductive factor. J. Biol. Chem. 265: 5024-5029, 1990). We now find that this fraction also contained small amounts of bone morphogenetic protein-2 and 3 (BMP-2 + 3) previously identified by others (see Wozney, J. M.: Bone morphogenetic proteins. Prog. Growth Factor Res. 1: 267-280, 1990). Separation of BMP-2 + 3 from the glycoprotein was achieved with a modified reversed phase-high pressure liquid chromatographic procedure. When assayed in the rat subcutis using a collagen-ceramic carrier, the osteoinductive activity was found in the subfraction containing BMP-2 + 3. This activity was potentiated and the ratio of cartilage to bone was increased by transforming growth factor-beta-2. The glycoprotein, originally called osteoinductive factor, has been renamed osteoglycin. In its precursor form, osteoglycin is a member of the leucine-rich family of proteins showing the characteristic 24-residue internal homology. Its biological function is unknown.

引用

页码：269 / 275

页数：7

共 30 条

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[2]

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[3]

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