ENHANCED MESSENGER-RNA CONTENT IN RESPONSE TO AMINO-ACID STARVATION FOR A 73 KDA PROTEIN OF THE INNER MITOCHONDRIAL-MEMBRANE

被引：5

作者：

CHILES, TC ^{[1
]}

LAINE, RO ^{[1
]}

SHAY, NF ^{[1
]}

HANDLOGTEN, ME ^{[1
]}

NICK, HS ^{[1
]}

KILBERG, MS ^{[1
]}

机构：

[1] UNIV FLORIDA, COLL MED, DEPT BIOCHEM & MOLEC BIOL, BOX 100245, GAINESVILLE, FL 32610 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 03期

关键词：

D O I：

10.1006/bbrc.1993.1734

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Biosynthesis of several rat liver proteins is enhanced by amino acid deprivation of cultured hepatocytes or hepatoma cells. One of these proteins, MP-73, was synthesized at a rate 2- to 3-fold greater when cells were incubated for 3-9 h under conditions of amino acid deprivation versus amino acid supplementation. Immunoblotting with polyclonal antibodies prepared against MP-73 localized it to the inner mitochondrial membrane. MP-73 appears to be a hydrophobic, integral membrane protein. MP-73 antibody was used to identify a partialy cDNA (NS3.2) of approximately 2 kb. A probe prepared from pNS3.2 identified a transcript in rat Fao hepatoma cells of approximately 4.4 kb that was increased in abundance by more than 20-fold following amino acid starvation of the cells. © 1993 Academic Press, Inc.

引用

页码：1068 / 1075

页数：8

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