INTRACELLULAR RECEPTOR-TYPE TRANSCRIPTION FACTOR, LASR, CONTAINS A HIGHLY CONSERVED AMPHIPATHIC REGION WHICH PRECEDES THE PUTATIVE HELIX-TURN-HELIX DNA-BINDING MOTIF

被引:11
作者
FUKUSHIMA, J [1 ]
ISHIWATA, T [1 ]
KURATA, M [1 ]
YOU, ZY [1 ]
OKUDA, K [1 ]
机构
[1] YOKOHAMA CITY UNIV,SCH MED,DEPT BACTERIOL,KANAZAWA KU,YOKOHAMA 236,JAPAN
关键词
D O I
10.1093/nar/22.18.3706
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have cloned and sequenced the lasR gene, which is involved in the trascriptional activation of several pathogenic factors, from Pseudomonas aeruginosa lFO3455 and PA103. These clones were predicted to be an open reading frame of 239 amino acids as reported for the PAO1 strain. There is only a single base change resulting in an amino acid exchange from M145 (PAO1) to I (lFO3455). PA103 DNA differs with PAO1 DNA in two bases resulting in only a single amino acid substitution from R179 to W. When the IFO3455 LasR was expressed in a PA103 strain which is known to be LasR defective, proteinase gene activation was detected, however, when PA103 LasR was expressed, no enhancement was measurable. From these results, it appears that the amino acid substitution of R179 to W inactivated LasR activity. This substitution is located in the highly conserved sequence found in many transcription factors, including a factors, and may disrupt amphipathic ct-helix, predicted for the 176 to 189 region, which precedes the putative helix - turn helix DNA binding motif. We presumed that these three helices may contribute to specific DNA binding.
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页码:3706 / 3707
页数:2
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