INVOLVEMENT OF PROTEIN-KINASE IN DELTA(12)-PROSTAGLANDIN J(2)-INDUCED EXPRESSION OF RAT HEME OXYGENASE-1 GENE

被引：18

作者：

NEGISHI, M ^{[1
]}

ODANI, N ^{[1
]}

KOIZUMI, T ^{[1
]}

TAKAHASHI, S ^{[1
]}

ICHIKAWA, A ^{[1
]}

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT PHYSIOL CHEM,SAKYO KU,KYOTO 606,JAPAN

来源：

FEBS LETTERS | 1995年 / 372卷 / 2-3期

关键词：

DELTA(12)-PROSTAGLANDIN J(2); HEME OXYGENASE; PROTEIN KINASE;

D O I：

10.1016/0014-5793(95)01001-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently identified the cis-regulatory element and its specific nuclear binding factors for Delta(12)-prostaglandin (PG) J(2)-induced expression of the rat heme oxygenase, HO-1 [Koizumi, T., Odani, N., Okuyama, T., Ichikawa, A. and Negishi, M. (1995) J. Biol. Chem. 270, in press]. Here we further examined the molecular mechanism underlying the Delta(12)-PGJ(2)-induced HO-1 gene expression. Protein kinase inhibitors, 2-aminopurine and staurosporine, suppressed the Delta(12)-PGJ(2)-induced HO-1 mRNA and the nuclear protein binding to the Delta(12)-PGJ(2)-responsive cis-regulatory element in rat basophilic leukemia cells. Furthermore, the nuclear protein binding to the element was suppressed by in vitro phosphatase treatment of the nuclear proteins from Delta(12)-PGJ(2)-treated cells. These findings suggest that Delta(12)-PGJ(2) induces the expression of the HO-1 gene through phosphorylation of the nuclear proteins which bind to the Delta(12)-PGJ(2)-responsive element.

引用

页码：279 / 282

页数：4

共 26 条

[1] IDENTIFICATION OF A 2ND REGION UPSTREAM OF THE MOUSE HEME OXYGENASE-1 GENE THAT FUNCTIONS AS A BASAL LEVEL AND INDUCER-DEPENDENT TRANSCRIPTION ENHANCER [J].

ALAM, J ;

CAMHI, S ;

CHOI, AMK .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (20) :11977-11984

[2] ANTIPROLIFERATIVE PROSTAGLANDINS ACTIVATE HEAT-SHOCK TRANSCRIPTION FACTOR [J].

AMICI, C ;

SISTONEN, L ;

SANTORO, MG ;

MORIMOTO, RI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (14) :6227-6231

[3]

BROCK TG, 1994, J BIOL CHEM, V269, P22059

[4] CELL-GROWTH INHIBITION BY PROSTAGLANDIN-A(2) RESULTS IN ELEVATED EXPRESSION OF GADD153 MESSENGER-RNA [J].

CHOI, AMK ;

FARGNOLI, J ;

CARLSON, SG ;

HOLBROOK, NJ .

EXPERIMENTAL CELL RESEARCH, 1992, 199 (01) :85-89

[5]

COX JS, 1993, CELL, V732, P1197

[6] HEAT-SHOCK PROTEINS AND MOLECULAR CHAPERONES - MEDIATORS OF PROTEIN CONFORMATION AND TURNOVER IN THE CELL [J].

CRAIG, EA ;

WEISSMAN, JS ;

HORWICH, AL .

CELL, 1994, 78 (03) :365-372

[7] BIOLOGICAL-ACTIVITIES AND MECHANISMS OF ACTION OF PGJ2 AND RELATED-COMPOUNDS - AN UPDATE [J].

FUKUSHIMA, M .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1992, 47 (01) :1-12

[8] THE REGULATION OF TRANSCRIPTION BY PHOSPHORYLATION [J].

HUNTER, T ;

KARIN, M .

CELL, 1992, 70 (03) :375-387

[9]

KAGEYAMA M, 1991, J PHARMACOL EXP THER, V259, P1019

[10]

KEYSE SM, 1989, CARCINOGENESIS, V11, P787

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