FAST INFERENTIAL ADAPTIVE OPTIMIZATION OF A CONTINUOUS YEAST CULTURE BASED ON CARBON-DIOXIDE EVOLUTION RATE

被引:9
作者
CHANG, YK
LIM, HC
机构
[1] UNIV CALIF IRVINE,BIOCHEM ENGN PROGRAM,IRVINE,CA 92717
[2] PURDUE UNIV,SCH CHEM ENGN,W LAFAYETTE,IN 47907
关键词
Cell Culture;
D O I
10.1002/bit.260350103
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A fast inferential, multivariable adaptive optimization algorithm based on a fast responding off‐gas data, the carbon dioxide evolution rate (CER), has been developed and applied to a continuous baker's yeast culture to maximize the cellular productivity in simulation and experimental studies. In the simulation study the process was optimized based on CER measurements using readily available steady‐state data on the ratio between the cellular productivity and the CER. It was shown that the algorithm is two to three times faster than the algorithm based on cell mass concentration measurements. In the experimental study the CER was maximized without any information on the relationship between the cellular productivity and the CER. It took about 40 h for the process to converge, while about 80 h was required when the optimization was based on cell mass measurements. The attained steady state was found to be different but fairly close to that obtained with cell measurements. Briefly discussed is a switching to the cell‐mass‐based algorithm at the final stage of the optimization to overcome a potential inaccuracy. Copyright © 1990 John Wiley & Sons, Inc.
引用
收藏
页码:8 / 14
页数:7
相关论文
共 8 条
[1]  
CHANG YK, 1988, UNPUB BIOTECHNOL BIO
[2]  
CHANG YK, 1987, THESIS PURDUE U W LA
[3]  
CHANG YK, 1988, BIOTECHNOL BIOENG, V31, P94
[4]   ADAPTIVE STEADY-STATE OPTIMIZATION OF BIOMASS PRODUCTIVITY IN CONTINUOUS FERMENTERS [J].
HARMON, J ;
SVORONOS, SA ;
LYBERATOS, G .
BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (03) :335-344
[5]  
HARMON J, 1985, 1985 P AM CONTR C BO, P685
[6]  
LIEVENSE J, 1984, THESIS PURDUE U W LA
[7]  
ROLF MJ, 1984, THESIS PURDUE U W LA
[8]  
SEMONES GB, 1986, THESIS PURDUE U W LA