GROWTH OF POLIOVIRUS USING ALGINATE ENTRAPPED-ANCHORAGE DEPENDENT VERO CELLS

被引：2

作者：

TREPANIER, P

DUGRE, R

ALAIN, R

LAPIERRE, J

机构：

[1] Division of Viral Vaccines, Institut Armand-Frappier, Laval-des-Rapides, H7N 4Z3, Québec

[2] Virus Research Centre, Institut Armand-Frappier, Laval-des-Rapides, H7N 4Z3, Québec

来源：

CYTOTECHNOLOGY | 1990年 / 4卷 / 03期

关键词：

ALGINATE; ENCAPSULATION; ENTRAPMENT; VERO CELLS; POLIOVIRUS;

D O I：

10.1007/BF00563786

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Obligatory anchorage dependant Vero cells were successfully grown in gelatinous-like macrocarriers made of calcium alginate. Entrapped single cells were immobilized within the polymerized alginate matrix divide to form large spherical clumps of cells. A cell density of 17 x 10(6) cells/ml of alginate with over 95% viability was obtained after 14 days in spinner flasks. When subjected of poliovirus type I infection, spherical masses of Vero cells progressively showed extensive cytopathic effect but remained entrapped in the alginate matrix of the macrocarriers. Virus was released into a cellular debris free-like supernatant and reached a peak titer of 10(8.0) TCID50/ml after 72 hours.

引用

页码：261 / 269

页数：9

共 6 条

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