REDUCTIVE BIOTRANSFORMATION BY WILD-TYPE AND MUTANT STRAINS OF SACCHAROMYCES-CEREVISIAE IN AQUEOUS-ORGANIC SOLVENT BIPHASIC SYSTEMS

被引：24

作者：

NIKOLOVA, P ^{[1
]}

WARD, OP ^{[1
]}

机构：

[1] UNIV WATERLOO,DEPT BIOL,WATERLOO N2L 3G1,ONTARIO,CANADA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 08期

关键词：

SACCHAROMYCES-CEREVISIAE; BENZYL ALCOHOL; REDUCTIVE BIOTRANSFORMATION; BIPHASIC SYSTEMS;

D O I：

10.1002/bit.260390809

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole cells of Saccharomyces cerevisiae catalyzed the conversion of benzaldehyde to benzyl alcohol in aqueous-organic biphasic media. Reaction rate increased dramatically as moisture content of the solvent was increased in the range 0% to 2%. The highest biotransformation rates were observed when hexane was used as organic solvent. Benzaldehyde was also converted to benzyl alcohol by a cell-free crude extract in biphasic systems containing hexane, although the rate of product formation was much lower. Mutant strains of S. cerevisiae lacking some or all of the ADH isoenzymes, ADH I, II, and III, manifested similar rates for bioconversion of benzaldehyde to benzyl alcohol in both aqueous and two-phase systems. In general, conversion rates observed in aqueous media were 2 to 3 times higher than those observed in hexane containing 2% moisture.

引用

页码：870 / 876

页数：7

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