STRUCTURAL ORGANIZATION OF THE GENE ENCODING THE HUMAN IRON-SULFUR SUBUNIT OF SUCCINATE-DEHYDROGENASE

被引：45

作者：

AU, HC ^{[1
]}

REAMROBINSON, D ^{[1
]}

BELLEW, LA ^{[1
]}

BROOMFIELD, PLE ^{[1
]}

SAGHBINI, M ^{[1
]}

SCHEFFLER, IE ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

来源：

GENE | 1995年 / 159卷 / 02期

关键词：

MITOCHONDRION; ELECTRON TRANSPORT CHAIN; COMPLEX II; RESPIRATION; LATE INTRONS;

D O I：

10.1016/0378-1119(95)00162-Y

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The iron-sulfur protein (Ip) subunit of succinate dehydrogenase (SDH and complex II) of the respiratory chain is highly conserved in evolution [Gould et al., Proc. Natl. Acad, Sci. USA 86 (1989) 1934-1938]. We have cloned the entire human Ip cDNA, as well as the Ip-encoding gene (SDH-B) from two genomic human libraries. The cDNA contains a coding sequence of 840 nt, flanked by a 5'-UTR of 133 nt and a 3'-UTR of 123 nt. The entire transcript is encoded by eight exons within approx. 40 kb, The seven introns range in size from 0.75 kb to >11 kb, and they appear to be of the 'late' intron class, Approx. 5 kb of upstream sequence was also cloned, and approx. 2.4 kb of the promoter region were sequenced and analyzed for consensus elements binding potential transcription factors and transcriptional activators.

引用

页码：249 / 253

页数：5

共 20 条

[1]

ACKRELL BAC, 1992, CHEM BIOCH FLAVOENZY, P229

[2] CHARACTERIZATION OF THE GENE ENCODING THE IRON-SULFUR PROTEIN SUBUNIT OF SUCCINATE-DEHYDROGENASE FROM DROSOPHILA-MELANOGASTER [J].