CYTOCHROME B-558 ALPHA-SUBUNIT CLONING AND EXPRESSION IN RAT AORTIC SMOOTH-MUSCLE CELLS

被引：94

作者：

FUKUI, T ^{[1
]}

LASSEGUE, B ^{[1
]}

KAI, H ^{[1
]}

ALEXANDER, RW ^{[1
]}

GRIENDLING, KK ^{[1
]}

机构：

[1] EMORY UNIV, DEPT MED, DIV CARDIOL, ATLANTA, GA 30322 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1995年 / 1231卷 / 03期

关键词：

VASCULAR SMOOTH MUSCLE; SUPEROXIDE ANION; NADPH OXIDASE; CYTOCHROME B-558; CDNA CLONING;

D O I：

10.1016/0005-2728(95)00098-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have shown that the NADPH oxidase participates in the generation of superoxide anion in non-phagocytic cells. Here we report the isolation and nucleotide sequence of a cDNA for the cytochrome b-558 alpha-subunit of the NADPH oxidase in rat vascular smooth muscle cells (VSMCs). The coding region of the cDNA was 93% homologous to mouse and 81% to human in nucleotide sequence and 96% homologous to mouse and 89% to human in the deduced amino acid sequence. Our results provide a tool with which to explore the mechanism of superoxide anion generation in rat VSMCs and other non-phagocytic cells.

引用

页码：215 / 219

页数：5

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