MYCOBACTERIUM-PARATUBERCULOSIS ANTIGEN-D - CHARACTERIZATION AND EVIDENCE THAT IT IS A BACTERIOFERRITIN

被引：33

作者：

BROOKS, BW ^{[1
]}

YOUNG, NM ^{[1
]}

WATSON, DC ^{[1
]}

ROBERTSON, RH ^{[1
]}

SUGDEN, EA ^{[1
]}

NIELSEN, KH ^{[1
]}

BECKER, SAWE ^{[1
]}

机构：

[1] NATL RES COUNCIL CANADA,DIV BIOL SCI,OTTAWA K1A 0R6,ONTARIO,CANADA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1991年 / 29卷 / 08期

关键词：

D O I：

10.1128/JCM.29.8.1652-1658.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By using a combination of agarose and polyacrylamide gel electrophoresis, Mycobacterium paratuberculosis antigen D was resolved from a crude sonicated preparation of the organism and characterized as a component with a molecular mass of approximately 400,000 Da. While this component was composed mainly of protein, with unusually high proportions of glutamic acid and leucine, it was resistant to digestion with a number of proteolytic enzymes. Structural detail revealed by electron microscopy, amino acid sequence data, and the demonstration of a Soret band in its absorption spectrum indicated that antigen D was similar to an Escherichia coli bacterioferritin.

引用

页码：1652 / 1658

页数：7

共 36 条

[1] AMINO-ACID SEQUENCE OF THE BACTERIOFERRITIN (CYTOCHROME-B1) OF ESCHERICHIA-COLI-K12
ANDREWS, SC
SMITH, JMA
GUEST, JR
HARRISON, PM
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 158 (02) : 489 - 496
[2] ELECTRON-DENSITY MAP OF APOFERRITIN AT 2.8-A RESOLUTION
BANYARD, SH
STAMMERS, DK
HARRISON, PM
[J]. NATURE, 1978, 271 (5642) : 282 - 284
[3] BECHNIELSEN S, 1985, AM J VET RES, V46, P2418
[4] BENNETT JC, 1967, METHOD ENZYMOL, V11, P211
[5] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6] BROOKS BW, 1988, 1988 C RES WORK AN D, V52, P199
[7] HEMOPROTEIN FROM AZOTOBACTER CONTAINING NON-HEME IRON - ISOLATION AND CRYSTALLIZATION
BULEN, WA
LECOMTE, JR
LOUGH, S
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1973, 54 (04) : 1274 - 1281
[8] CHIODINI RJ, 1984, CORNELL VET, V74, P218
[9] IDENTIFICATION OF 2 GROUPS OF MYCOBACTERIUM-PARATUBERCULOSIS STRAINS BY RESTRICTION ENDONUCLEASE ANALYSIS AND DNA HYBRIDIZATION
COLLINS, DM
GABRIC, DM
DELISLE, GW
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (07) : 1591 - 1596
[10] GRUFT H, 1978, AM REV RESPIR DIS, V68, P179

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