SELECTIVE INDUCTION OF INTERCELLULAR-ADHESION MOLECULE-1 BY INTERFERON-GAMMA IN HUMAN AIRWAY EPITHELIAL-CELLS

被引:171
作者
LOOK, DC
RAPP, SR
KELLER, BT
HOLTZMAN, MJ
机构
[1] WASHINGTON UNIV,SCH MED,DEPT MED,660 S EUCLID AVE,BOX 8052,ST LOUIS,MO 63110
[2] MONSANTO CO,MONSANTO CORP RES,DEPT HLTH SCI,ST LOUIS,MO 63167
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1992年 / 263卷 / 01期
关键词
POLYMORPHONUCLEAR LEUKOCYTE ADHERENCE; INFLAMMATION; VASCULAR ENDOTHELIAL CELLS; INTERLEUKIN-1-BETA; TUMOR NECROSIS FACTOR-ALPHA;
D O I
10.1152/ajplung.1992.263.1.L79
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To evaluate the factors controlling migration of leukocytes into pulmonary airway epithelium, we determined the biochemical mechanisms responsible for the regulation of intercellular adhesion molecule-1 (ICAM-1) expression on cultured monolayers of human tracheal epithelial cells (HTECs) or SV40 virus-transformed human bronchial epithelial cells (BEAS-2B). Validation experiments with human umbilical vein endothelial cells (HUVECs) demonstrated little detectable ICAM-1 expression on unstimulated cells or on cells incubated with interferon-gamma (IFN-gamma), but HUVEC monolayers responded to interleukin-1-beta (IL-1-beta) or tumor necrosis factor-alpha (TNF-alpha) with significant increases in ICAM-1 and ICAM-1-dependent adherence of polymorphonuclear leukocytes (PMNs). HTEC monolayers also exhibited no significant basal ICAM-1 expression but, in contrast to HUVEC monolayers, had marked increases in ICAM-1 expression and ICAM-1-dependent PMN adherence only after incubation with IFN-gamma (and not after IL-1-beta or TNF-alpha) treatment. BEAS-2B cells also exhibited relatively selective IFN-gamma stimulation of ICAM-1 expression and ICAM-1-dependent PMN adherence but (like late passage HTEC) showed significant basal ICAM-1 expression. Differences in IFN-gamma effect on ICAM-1 levels between HUVEC and HTEC monolayers were not due to differences in number or responsiveness of IFN-gamma receptors, because both cell types exhibited a similar number of receptors and other IFN-gamma-dependent responses of HUVECs remained active. In all analyses, ICAM-1 mRNA levels correlated closely with detection of ICAM-1 on the cell surface. The results indicate that ICAM-1 is expressed on human airway epithelial cells and that ICAM-1-dependent mechanisms may mediate PMN adherence to these cells. As in other cell types, induction of ICAM-1 in airway epithelial cells appears to be transcriptionally regulated, but in contrast to endothelial cells, the induction of ICAM-1 in airway epithelial cells is selectively responsive to IFN-gamma.
引用
收藏
页码:L79 / L87
页数:9
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