AN IMPROVED LONG-TERM CULTURE OF RAT HEPATOCYTES TO DETECT LIVER TUMOR-PROMOTING AGENTS - RESULTS WITH PHENOBARBITAL

被引:34
作者
MESNIL, M
PICCOLI, C
YAMASAKI, H
机构
[1] International Agency for Research on Cancer, 69372 Lyon Cedex 08
来源
EUROPEAN JOURNAL OF PHARMACOLOGY-ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY SECTION | 1993年 / 248卷 / 01期
关键词
LIVER TUMOR PROMOTION (RAT); HEPATOCYTES; GAP-JUNCTIONAL INTERCELLULAR COMMUNICATION; DYE TRANSFER; CONNEXIN-32; (LONG-TERM COCULTURE);
D O I
10.1016/0926-6917(93)90025-L
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Among various cocultures of hepatocytes with other cell types, we found that mouse embryonal cells (BALB/c 3T3) are more effective in maintaining rat hepatocytes in vitro. Because most human cancers are epithelial in origin, we thought that such a hepatocyte culture system could be used for the detection of tumour-promoting agents, most of which are inhibitors of gap-junctional intercellular communication. We, therefore, have examined the effect of the strong rat liver tumour promoter, phenobarbital, on the gap-junctional intercellular communication capacity of hepatocytes in long-term cultures. A single application of phenobarbital drastically inhibited the gap-junctional intercellular communication between hepatocytes in a coculture for only several hours, but treatment for 3 weeks provoked a constant decrease of gap-junctional intercellular communication (50%) throughout the treatment period. This type of long-term culture of rat hepatocytes may be usable in a rapid in vitro assay to detect tumour-promoting agents.
引用
收藏
页码:59 / 66
页数:8
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