MYOSIN LIGHT-CHAIN KINASE OCCURS IN BULLFROG SYMPATHETIC NEURONS AND MAY MODULATE VOLTAGE-DEPENDENT POTASSIUM CURRENTS

被引：41

作者：

AKASU, T

ITO, M

NAKANO, T

SCHNEIDER, CR

SIMMONS, MA

TANAKA, T

TOKIMASA, T

YOSHIDA, M

机构：

[1] KURUME UNIV,SCH MED,DEPT PHARMACOL,KURUME,FUKUOKA 830,JAPAN

[2] MIE UNIV,DEPT INTERNAL MED,TSU,MIE 514,JAPAN

[3] MIE UNIV,DEPT MOLEC & CELLULAR PHARMACOL,TSU,MIE 514,JAPAN

[4] MARSHALL UNIV,DEPT PHARMACOL,NEUROPHARMACOL LAB,HUNTINGTON,WV 25755

来源：

NEURON | 1993年 / 11卷 / 06期

关键词：

D O I：

10.1016/0896-6273(93)90226-H

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A polyclonal antibody against myosin light chain kinase (MLCK) of chicken gizzard recognized a 130 kd peptide of bullfrog sympathetic ganglia as MLCK. MLCK immunoreactivity was confined to the neuronal cell body. A synthetic peptide corresponding to an inhibitory domain of MLCK (Ala783-Gly804) Was applied intracellularly to isolated sympathetic neurons during whole-cell recordings of ionic currents. The peptide inhibitor reversibly decreased M-type potassium current (I(M)) while not affecting A-type or delayed rectifier-type potassium currents. Intracellular application of an active fragment of MLCK enhanced I(M), whereas application of an inactive MLCK fragment did not. The results suggest that I(M) can be modulated by MLCK-catalyzed phosphorylation.

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收藏

页码：1133 / 1145

页数：13

相关论文

共 45 条

[1] M-CURRENTS AND OTHER POTASSIUM CURRENTS IN BULLFROG SYMPATHETIC NEURONS [J].

ADAMS, PR ;

BROWN, DA ;

CONSTANTI, A .

JOURNAL OF PHYSIOLOGY-LONDON, 1982, 330 (SEP) :537-572

[2] PROTEIN KINASE-C IS NOT NECESSARY FOR PEPTIDE-INDUCED SUPPRESSION OF M-CURRENT OR FOR DESENSITIZATION OF THE PEPTIDE RECEPTORS [J].

BOSMA, MM ;

HILLE, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (08) :2943-2947

[3] MUSCARINIC SUPPRESSION OF A NOVEL VOLTAGE-SENSITIVE K+ CURRENT IN A VERTEBRATE NEURON [J].

BROWN, DA ;

ADAMS, PR .

NATURE, 1980, 283 (5748) :673-676

[4] EFFECTS OF PHORBOL DIBUTYRATE ON M-CURRENTS AND M-CURRENT INHIBITION IN BULLFROG SYMPATHETIC NEURONS [J].

BROWN, DA ;

ADAMS, PR .

CELLULAR AND MOLECULAR NEUROBIOLOGY, 1987, 7 (03) :255-269

[5] THE NAPHTHALENESULPHONAMIDE CALMODULIN ANTAGONIST W7 AND ITS 5-IODO-1-C8 ANALOG INHIBIT POTASSIUM AND CALCIUM CURRENTS IN NG108-15 NEUROBLASTOMA-X GLIOMA-CELLS IN A MANNER POSSIBLY UNRELATED TO THEIR ANTAGONISM OF CALMODULIN [J].

CAULFIELD, MP ;

ROBBINS, J ;

SIM, JA ;

BROWN, DA ;

MACNEIL, S ;

BLACKBURN, GM .

NEUROSCIENCE LETTERS, 1991, 125 (01) :57-61

[6]

GLASS DB, 1989, J BIOL CHEM, V264, P8802

[7] INFLUENCE OF TEMPERATURE UPON CONTRACTILE ACTIVATION AND ISOMETRIC FORCE PRODUCTION IN MECHANICALLY SKINNED MUSCLE-FIBERS OF THE FROG [J].

GODT, RE ;

LINDLEY, BD .

JOURNAL OF GENERAL PHYSIOLOGY, 1982, 80 (02) :279-297

[8] DOMAIN ORGANIZATION OF CHICKEN GIZZARD MYOSIN LIGHT CHAIN KINASE DEDUCED FROM A CLONED CDNA [J].

GUERRIERO, V ;

RUSSO, MA ;

OLSON, NJ ;

PUTKEY, JA ;

MEANS, AR .

BIOCHEMISTRY, 1986, 25 (26) :8372-8381

[9] PRODUCTION AND CHARACTERIZATION OF AN ANTIBODY TO MYOSIN LIGHT CHAIN KINASE AND INTRACELLULAR-LOCALIZATION OF THE ENZYME [J].

GUERRIERO, V ;

ROWLEY, DR ;

MEANS, AR .

CELL, 1981, 27 (03) :449-458

[10] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

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