GLUTATHIONE-PEROXIDASE ISOLATED FROM PLASMA REDUCES PHOSPHOLIPID HYDROPEROXIDES

被引：131

作者：

YAMAMOTO, Y ^{[1
]}

TAKAHASHI, K ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,DEPT HYG CHEM,KITA 16,NISHI 6,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1993年 / 305卷 / 02期

关键词：

D O I：

10.1006/abbi.1993.1458

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The reactivities of a selenoenzyme, glutathione peroxidase isolated from plasma, against phosphatidylcholine hydroperoxides and photoperoxidized erythrocyte ghosts have been investigated. Glutathione peroxidase isolated from plasma was found to catalyze the reduction of phosphatidylcholine hydroperoxides to the corresponding hydroxy derivatives. The enzyme is also capable of reducing the hydroperoxides in photoperoxidized ghosts. Thin layer chromatographic analysis of enzyme-treated ghosts revealed that plasma glutathione peroxidase can reduce phospholipid-derived hydroperoxides but cannot reduce cholesterol hydroperoxides. These studies demonstrate that the three known glutathione peroxidase (cellular, plasma, and phospholipid hydroperoxide) differ from each other in substrate specificity. © 1993 Academic Press, Inc.

引用

页码：541 / 545

页数：5

共 24 条

[1] AWASTHI YC, 1975, J BIOL CHEM, V250, P5144
[2] DUAN YJ, 1988, J BIOL CHEM, V263, P19003
[3] THE REFINED STRUCTURE OF THE SELENOENZYME GLUTATHIONE-PEROXIDASE AT 0.2-NM RESOLUTION
EPP, O
LADENSTEIN, R
WENDEL, A
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 133 (01): : 51 - 69
[4] CHARACTERIZATION AND PARTIAL AMINO-ACID-SEQUENCE OF HUMAN PLASMA GLUTATHIONE-PEROXIDASE
ESWORTHY, RS
CHU, FF
PAXTON, RJ
AKMAN, S
DOROSHOW, JH
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 286 (02) : 330 - 336
[5] FLOHE L, 1992, 5TH INT S SEL BIOL M, P21
[6] Flohe L., 1989, GLUTATHIONE, P644
[7] IDENTIFICATION OF CATALYTIC SITE OF RAT-LIVER GLUTATHIONE PEROXIDASE AS SELENOCYSTEINE
FORSTROM, JW
ZAKOWSKI, JJ
TAPPEL, AL
[J]. BIOCHEMISTRY, 1978, 17 (13) : 2639 - 2644
[8] ANTIOXIDANT DEFENSES AND LIPID-PEROXIDATION IN HUMAN-BLOOD PLASMA
FREI, B
STOCKER, R
AMES, BN
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9748 - 9752
[9] LIPID PHOTOOXIDATION IN ERYTHROCYTE-GHOSTS - SENSITIZATION OF THE MEMBRANES TOWARD ASCORBATE-INDUCED AND SUPEROXIDE-INDUCED PEROXIDATION AND LYSIS
GIROTTI, AW
THOMAS, JP
JORDAN, JE
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1985, 236 (01) : 238 - 251
[10] MADDIPATI KR, 1987, J BIOL CHEM, V262, P17398

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