ROLE FOR A SYNAPSE-SPECIFIC CARBOHYDRATE IN AGRIN-INDUCED CLUSTERING OF ACETYLCHOLINE-RECEPTORS

被引:75
作者
MARTIN, PT
SANES, JR
机构
[1] Department of Anatomy, Neurobiology Washington University School of Medicine St. Louis
基金
美国国家卫生研究院;
关键词
D O I
10.1016/0896-6273(95)90218-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Lectins such as VVA-B-4, which bind N-acetylgalactosaminyl (GalNAc)-terminated saccharides, selectively stain the neuromuscular junction, thus defining a synapse-specific carbohydrate. In seeking roles for this carbohydrate, we asked whether VVA-B-4 affected the distribution of acetylcholine receptors (AChRs) on cultured muscle cells. We found that incubation of myotubes with VVA-B-4 induced formation of AChR clusters and potentiated the effect of a nerve-derived clustering factor, agrin. Additional experiments implicated GalNAc-terminated glycoconjugates as modulators of agrin-induced AChR clustering. Enzymatic removal of GalNAc residues or treatment with a multivalent protein-GalNAc conjugate blocked agrin-induced clustering, whereas enzymatic unmasking of additional GalNAc residues induced clustering in the absence of added agrin. Moreover, incubation with agrin led to redistribution of VVA-B-4-binding material on myotubes. Together, these results suggest that agrin-induced clustering of AChRs involves a GalNAc-dependent step.
引用
收藏
页码:743 / 754
页数:12
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