A DNA TOPOISOMERASE-I INHIBITOR BLOCKS THE DIFFERENTIATION OF RAT GRANULOSA-CELLS INDUCED BY FOLLICLE-STIMULATING-HORMONE

被引：8

作者：

BARANAO, JL

BLEY, MA

BATISTA, FD

GLIKIN, GC

机构：

[1] UNIV BUENOS AIRES, FAC CIENCIAS EXACTAS & NAT, RA-1428 BUENOS AIRES, ARGENTINA

[2] UNIV BUENOS AIRES, CONSEJO NACL INVEST CIENT & TECN, RA-1428 BUENOS AIRES, ARGENTINA

来源：

BIOCHEMICAL JOURNAL | 1991年 / 277卷

关键词：

D O I：

10.1042/bj2770557

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Follicle-stimulating hormone (FSH), acting through a cyclic AMP-mediated mechanism, promotes differentiation of rat granulosa cells cultured in a defined medium. Camptothecin, a DNA topoisomerase I blocker, inhibited the increase in progesterone and oestradiol production stimulated by FSH. This effect was not due to non-specific inhibition of protein synthesis, as shown by measurement of [S-35]methionine incorporation. A transient increase in DNA topoisomerase I activity was observed after 24 h of culture in the presence of FSH or dibutyryl cyclic AMP. Our results are consistent with a key role for DNA topoisomerase I in the modulation of gene expression by FSH in rat granulosa cells.

引用

页码：557 / 560

页数：4

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