QUANTITATION OF A LENTIVIRUS IN ITS NATURAL HOST - SIMIAN IMMUNODEFICIENCY VIRUS IN AFRICAN-GREEN MONKEYS

We have examined the viral load in the peripheral blood of simian immunodeficiency virus (SIV)-infected African green monkeys with a view to the unexplained apathogenicity of African green monkey SIV (SIV(agm)) in its natural host. By using polymerase chain reaction, viral DNA was detected in fresh peripheral blood mononuclear cells (PBMC) of each of nine seropositive animals. The virus DNA load was variable among the monkeys tested, ranging from 5 to 50 (mean = 15) copies per 10(5) PBMC, which is comparable to that of human immunodeficiency virus type 1 (HIV-1) in humans. The level of infectious SIV(agm) in PBMC was measured by endpoint dilution cultures. SIV(agm) was recovered from PBMC from 14 of 17 antibody-positive monkeys (82%), and the mean SIV(agm) titer in PBMC of seropositive African green monkeys was 10 tissue culture infectious doses per 10(6) cells, similar to the titer shown for HIV in asymptomatic carriers. Free infectious virus was isolated from the plasma of 4 of 17 monkeys (24%), and SIV(agm) expression in peripheral blood in vivo, as demonstrated by in situ hybridization, was detectable only in those animals which were viremic. SIV(agm) replication is therefore not totally suppressed in vivo, and SIV(agm) has a viral load equivalent to that seen for HIV-1 in asymptomatic humans.