EFFECTS OF SELECTED SECONDARY METABOLITES OF FUSARIUM-MONILIFORME ON UNSCHEDULED SYNTHESIS OF DNA BY RAT PRIMARY HEPATOCYTES

被引：82

作者：

NORRED, WP ^{[1
]}

PLATTNER, RD ^{[1
]}

VESONDER, RF ^{[1
]}

BACON, CW ^{[1
]}

VOSS, KA ^{[1
]}

机构：

[1] USDA ARS,NO CTR AGR UTILIZAT RES,PEORIA,IL 61604

来源：

FOOD AND CHEMICAL TOXICOLOGY | 1992年 / 30卷 / 03期

关键词：

D O I：

10.1016/0278-6915(92)90038-M

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The Fusarium moniliforme mycotoxins-fusarin C, fumonisin B1, moniliformin and bikaverin-were evaluated for genotoxicity by their ability to induce unscheduled DNA synthesis (UDS) in primary rat hepatocytes. Isolated hepatocytes were exposed to several concentrations of moniliformin (5.0-500-mu-M), bikaverin (1.0-500-mu-m), fumonisin B1 (0.5-250-mu-M), or fusarin C (1.0-100-mu-M). Aflatoxin B1, a known inducer of UDS, was included as a positive control. UDS was determined by autoradiography of cells after their exposure to [H-3]thymidine. The highest doses of fusarin C and bikaverin caused cell death, but no cytotoxicity was observed in cells exposed to moniliformin or fumonisin B1. Fumonisin B1, moniliformin and bikaverin were not genotoxic in the UDS assay. The results of the UDS assay with fusarin C were inconclusive since a marginal effect on UDS was obtained.

引用

页码：233 / 237

页数：5

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