DIRECT TRANSFER OF MEMBRANE-PROTEINS FROM B16 MELANOMA CELL TO ARTIFICIAL CELL LIPOSOME

Direct transfer of membrane proteins of B16 melanoma cell to lecithin liposome has been tried in two different cell culturing methods; namely, monolayer and suspension culture systems. For this purpose an appropriate amount of an artificial boundary lipid, 1,2-dimyristoylamido-1,2-deoxyphosphatidylcholine (DDPC), was added to 1,2-dimyristoylphosphatidylcholine (DMPC) liposome. An increase in the amount of DDPC to DMPC led to an increase in both the total amount and the kind of proteins transferred from the cell to the liposome. In addition, the amount and kind of proteins were differred also by altering the method of cell culture. These results indicate that the location and the mobility of the proteins in cytoplasm membrane are largely affected by the situation of the cells whether the cell adheres on a substrate or suspends in a medium.