IMMUNOCYTOCHEMICAL LOCALIZATION OF CYCLIN PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IN FERTILIZED-EGGS OF MICE

被引：2

作者：

BARAN, V

VESELA, J

REHAK, P

KOPPEL, J

机构：

[1] Slovak Academy of Sciences, Institute of Animal Physiology, Kosice

来源：

REPRODUCTION NUTRITION DEVELOPMENT | 1992年 / 32卷 / 03期

关键词：

CYCLIN; PCNA; DNA SYNTHESIS; IMMUNOCYTOCHEMISTRY; FERTILIZED MOUSE EGG;

D O I：

10.1051/rnd:19920303

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Immunolocalization of cyclin/PCNA (proliferating cell nuclear antigen) was performed with monoclonal antibody using immunogold methods on ultrathin cryosections of fertilized mouse eggs. Immunolabeling in pronuclei was checked 20, 22, 24 and 26 h after HCG injection. A relation between onset of pronuclei migration (early S-phase) and appearance of colloidal particle clusters was found. Afterwards, (mid S-phase) the increase of labelling and the localization of cyclin/PCNA were found throughout the pronuclei, except in the nucleolar bodies. Lower labelling appeared at the time of close reciprocal pronuclei contact (late S-phase). It is concluded that bulk and distribution of cyclin/PCNA in pronuclei is closely related to the progression of first interphase after fertilization.

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页码：241 / 248

页数：8

相关论文

共 17 条

[1] SYNTHESIS OF THE NUCLEAR-PROTEIN CYCLIN (PCNA) AND ITS RELATIONSHIP WITH DNA-REPLICATION [J].

BRAVO, R .

EXPERIMENTAL CELL RESEARCH, 1986, 163 (02) :287-293

[2] CHANGES IN THE NUCLEAR-DISTRIBUTION OF CYCLIN (PCNA) BUT NOT ITS SYNTHESIS DEPEND ON DNA-REPLICATION [J].

BRAVO, R ;

MACDONALDBRAVO, H .

EMBO JOURNAL, 1985, 4 (03) :655-661

[3] CYCLIN PCNA IS THE AUXILIARY PROTEIN OF DNA POLYMERASE-DELTA [J].

BRAVO, R ;

FRANK, R ;

BLUNDELL, PA ;

MACDONALDBRAVO, H .

NATURE, 1987, 326 (6112) :515-517

[4] IDENTIFICATION OF A NUCLEAR AND OF A CYTOPLASMIC POLYPEPTIDE WHOSE RELATIVE PROPORTIONS ARE SENSITIVE TO CHANGES IN THE RATE OF CELL-PROLIFERATION [J].

BRAVO, R ;

FEY, SJ ;

BELLATIN, J ;

LARSEN, PM ;

AREVALO, J ;

CELIS, JE .

EXPERIMENTAL CELL RESEARCH, 1981, 136 (02) :311-319

[5] EXISTENCE OF 2 POPULATIONS OF CYCLIN PROLIFERATING CELL NUCLEAR ANTIGEN DURING THE CELL-CYCLE - ASSOCIATION WITH DNA-REPLICATION SITES [J].

BRAVO, R ;

MACDONALDBRAVO, H .

JOURNAL OF CELL BIOLOGY, 1987, 105 (04) :1549-1554

[6]

BURKI K, 1986, EXPT EMBRYOLOGY MOUS

[7] CELL CYCLE-DEPENDENT VARIATIONS IN THE DISTRIBUTION OF THE NUCLEAR-PROTEIN CYCLIN PROLIFERATING CELL NUCLEAR ANTIGEN IN CULTURED-CELLS - SUBDIVISION OF S-PHASE [J].

CELIS, JE ;

CELIS, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (10) :3262-3266

[8] NUCLEAR PATTERNS OF CYCLIN (PCNA) ANTIGEN DISTRIBUTION SUBDIVIDE S-PHASE IN CULTURED-CELLS - SOME APPLICATIONS OF PCNA ANTIBODIES [J].

CELIS, JE ;

MADSEN, P ;

NIELSEN, S ;

CELIS, A .

LEUKEMIA RESEARCH, 1986, 10 (03) :237-249

[9] DNA POLYMERASE-DELTA - A 2ND EUKARYOTIC DNA REPLICASE [J].

DOWNEY, KM ;

TAN, CK ;

SO, AG .

BIOESSAYS, 1990, 12 (05) :231-236

[10] ON THE PREPARATION OF CRYOSECTIONS FOR IMMUNOCYTOCHEMISTRY [J].

GRIFFITHS, G ;

MCDOWALL, A ;

BACK, R ;

DUBOCHET, J .

JOURNAL OF ULTRASTRUCTURE RESEARCH, 1984, 89 (01) :65-78