HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY THERMOSPRAY MASS-SPECTROMETRY OF EPOXY POLYUNSATURATED FATTY-ACIDS AND EPOXYHYDROXY POLYUNSATURATED FATTY-ACIDS FROM AN INCUBATION MIXTURE OF RAT-TISSUE HOMOGENATE

被引：15

作者：

YAMANE, M ^{[1
]}

ABE, A ^{[1
]}

YAMANE, S ^{[1
]}

机构：

[1] TOHO UNIV,OHMORI HOSP,SCH MED,DEPT LAB MED,OHTA KU,TOKYO 143,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 652卷 / 02期

关键词：

D O I：

10.1016/0378-4347(93)E0394-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the analysis of epoxy polyunsaturated fatty acids (EpPUFAs) and epoxyhydroxy polyunsaturated fatty acids (EpHPUFAs) in rat tissue homogenate, with homo-gamma-linolenic acid (20:3, n - 6), arachidonic acid (20:4, n - 6), eicosapentaenoic acid (20:5, n - 3) or docosahexaenoic acid (22:6, n - 3) as a substrate, has been developed. Extraction with dichloromethane at pH 4-5 and concentration in the presence of pyridine were performed. Spectral analysis of chromatograms obtained with high-performance liquid chromatography-thermospray mass spectrometry showed the presence of EpPUFAs, EpHPUFAs and dihydroxy metabolites (DiHPUFAs) of EpPUFAs corresponding to each precursor fatty acid. On a selected-ion monitoring chromatogram, many EpPUFAs, EpHPUFAs and DiHPUFAs in an extract from an incubation mixture of each precursor fatty acid in aged rat tissue homogenate were detected simultaneously within 70 min. EpPUFAs and DiHPUFAs derived from 20:3 (n - 6) or 20:5 (n - 3) were detected in significant amounts. From these results, a highly active cytochrome P450 system or non-enzymic oxidative reactions in aged rat tissue homogenate were suggested.

引用

页码：123 / 136

页数：14

共 36 条

[1] CHARACTERIZATION OF EPOXIDES OF POLY-UNSATURATED FATTY-ACIDS BY MASS-SPECTROMETRY VIA 3-PYRIDINYLMETHYL ESTERS [J].

BALAZY, M ;

NIES, AS .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1989, 18 (05) :328-336

[2]

BALAZY M, 1991, J BIOL CHEM, V266, P23561

[3] FORMATION OF CIS-14,15-OXIDO-5,8,11-ICOSATRIENOIC ACID FROM PHOSPHATIDYLINOSITOL IN HUMAN-PLATELETS [J].

BALLOU, LR ;

LAM, BK ;

WONG, PYK ;

CHEUNG, WY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (20) :6990-6994

[4] COLLISIONALLY INDUCED DISSOCIATION OF EPOXYEICOSATRIENOIC ACIDS AND EPOXYEICOSATRIENOIC ACID PHOSPHOLIPID MOLECULAR-SPECIES [J].

BERNSTROM, K ;

KAYGANICH, K ;

MURPHY, RC .

ANALYTICAL BIOCHEMISTRY, 1991, 198 (01) :203-211

[5]

BRYANT RW, 1985, J BIOL CHEM, V260, P3548

[6]

CAPDEVILA J, 1983, ENDOCRINOLOGY, V113, P421

[7] CYTOCHROME P-450-DEPENDENT OXYGENATION OF ARACHIDONIC-ACID TO HYDROXYICOSATETRAENOIC ACIDS [J].

CAPDEVILA, J ;

MARNETT, LJ ;

CHACOS, N ;

PROUGH, RA ;

ESTABROOK, RW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (03) :767-770

[8]

CHUNG S, 1974, Tetrahedron Letters, V35, P3023

[9] ACTIVE METABOLISM OF ARACHIDONIC-ACID BY KAPOSI-SARCOMA CELLS CULTURED FROM LUNG BIOPSIES (KS-3) - IDENTIFICATION BY HPLC AND MS/MS OF THE PREDOMINANT METABOLITE SECRETED AS THE 11,12-EPOXY-EICOSATRIENOIC ACID [J].

DRAY, F ;

VULLIEZLENORMAND, B ;

DEROUSSENT, A ;

BRIQUET, I ;

GABELLEC, MM ;

NAKAMURA, S ;

WAHL, LM ;

GOUYETTE, A ;

SALAHUDDIN, ZS .

BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1180 (01) :83-90

[10]

FALCK JR, 1987, J LIPID RES, V28, P846

← 1 2 3 4 →