REPRESSION OF NITROGEN-FIXATION IN KLEBSIELLA-PNEUMONIAE AT HIGH-TEMPERATURE

被引：25

作者：

BROOKS, SJ

COLLINS, JJ

BRILL, WJ

机构：

[1] UNIV WISCONSIN, DEPT BACTERIOL, MADISON, WI 53706 USA

[2] UNIV WISCONSIN, CTR STUDIES NITROGEN FIXAT, MADISON, WI 53706 USA

来源：

JOURNAL OF BACTERIOLOGY | 1984年 / 157卷 / 02期

关键词：

D O I：

10.1128/JB.157.2.460-464.1984

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The effect of elevated growth temperature on nif mRNA synthesis, nif protein synthesis and nitrogenase activity was investigated in wild-type and NifL- strains of K. pneumoniae. Nitrogenase activity is not affected at 41.degree. C; however, nitrogenase is not synthesized at that temperature. Transcription of 3 nif operons studied is repressed at 41.degree. C. Apparently, the nifL protein is not required for repression by high temperature and repression of nif at 41.degree. C results from a reversible inactivation of the nifA gene product.

引用

收藏

页码：460 / 464

页数：5

相关论文

共 30 条

[1] USE OF BACTERIOPHAGE-MU TO ISOLATE DELETIONS IN HIS-NIF REGION OF KLEBSIELLA-PNEUMONIAE [J].

BACHHUBER, M ;

BRILL, WJ ;

HOWE, MM .

JOURNAL OF BACTERIOLOGY, 1976, 128 (03) :749-753

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] ROLE OF THE NIFA GENE-PRODUCT IN THE REGULATION OF NIF EXPRESSION IN KLEBSIELLA-PNEUMONIAE [J].

BUCHANANWOLLASTON, V ;

CANNON, MC ;

BEYNON, JL ;

CANNON, FC .

NATURE, 1981, 294 (5843) :776-778

[4] ANALYSIS OF REGULATION OF KLEBSIELLA-PNEUMONIAE NITROGEN-FIXATION (NIF) GENE-CLUSTER WITH GENE FUSIONS [J].

DIXON, R ;

EADY, RR ;

ESPIN, G ;

HILL, S ;

IACCARINO, M ;

KAHN, D ;

MERRICK, M .

NATURE, 1980, 286 (5769) :128-132

[5] COMPLEMENTATION ANALYSIS OF KLEBSIELLA-PNEUMONIAE MUTANTS DEFECTIVE IN NITROGEN-FIXATION [J].

DIXON, R ;

KENNEDY, C ;

KONDOROSI, A ;

KRISHNAPILLAI, V ;

MERRICK, M .

MOLECULAR & GENERAL GENETICS, 1977, 157 (02) :189-198

[6] POSITIVE CONTROL AND AUTOGENOUS REGULATION OF THE NIFLA PROMOTER IN KLEBSIELLA-PNEUMONIAE [J].

DRUMMOND, M ;

CLEMENTS, J ;

MERRICK, M ;

DIXON, R .

NATURE, 1983, 301 (5898) :302-307

[7] CLONING OF THE GLNA, NTRB AND NTRC GENES OF KLEBSIELLA-PNEUMONIAE AND STUDIES OF THEIR ROLE IN REGULATION OF THE NITROGEN-FIXATION (NIF) GENE-CLUSTER [J].

ESPIN, G ;

ALVAREZMORALES, A ;

CANNON, F ;

DIXON, R ;

MERRICK, M .

MOLECULAR & GENERAL GENETICS, 1982, 186 (04) :518-524

[8] A RAPID AND EFFICIENT PROCEDURE FOR THE PURIFICATION OF DNA FROM AGAROSE GELS [J].

GIRVITZ, SC ;

BACCHETTI, S ;

RAINBOW, AJ ;

GRAHAM, FL .

ANALYTICAL BIOCHEMISTRY, 1980, 106 (02) :492-496

[9] TEMPERATURE CONTROL OF NITROGEN-FIXATION IN KLEBSIELLA-PNEUMONIAE [J].

HENNECKE, H ;

SHANMUGAM, KT .

ARCHIVES OF MICROBIOLOGY, 1979, 123 (03) :259-265

[10] NITROGEN-FIXATION GENE (NIFL) INVOLVED IN OXYGEN REGULATION OF NITROGENASE SYNTHESIS IN K-PNEUMONIAE [J].

HILL, S ;

KENNEDY, C ;

KAVANAGH, E ;

GOLDBERG, RB ;

HANAU, R .

NATURE, 1981, 290 (5805) :424-426