Biolistic transformation of the obligate plant pathogenic fungus, Erysiphe graminis f sp hordei

被引：23

作者：

Christiansen, SK ^{[1
]}

Knudsen, S ^{[1
]}

Giese, H ^{[1
]}

机构：

[1] CARLSBERG RES LAB,DK-2500 VALBY,DENMARK

来源：

CURRENT GENETICS | 1995年 / 29卷 / 01期

关键词：

promoter; particle gun; GUS;

D O I：

10.1007/BF00313200

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Particle gun acceleration appears to be a possible way to transform mycelium cells of obligate plant parasites growing on host surfaces, GUS expression was obtained in E. graminis f.sp. hordei cells after bombardment with the GUS gene under the control of the E. graminis f.sp. hordei beta-tubulin promoter. Three heterologous promoters, one from Aspergillus nidulans and two from Cochliobolus heterostrophus, gave very low or no expression of GUS.

引用

页码：100 / 102

页数：3

共 16 条

[1]

BHARI SM, 1992, PHYTOPATHOLOGY, V82, P986

[2] TRANSFORMATION OF THE FUNGAL PATHOGEN CRYPHONECTRIA-PARASITICA WITH A VARIETY OF HETEROLOGOUS PLASMIDS [J].