PHOSPHORYLATION OF ORNITHINE DECARBOXYLASE IN INTACT ERYTHROLEUKEMIA-CELLS

被引：14

作者：

FLAMIGNI, F

MARMIROLI, S

MEGGIO, F

GUARNIERI, C

PINNA, LA

机构：

[1] UNIV PADUA,DEPARTIMENTO CHIM BIOL,VIA TRIESTE 75,I-35131 PADUA,ITALY

[2] UNIV BOLOGNA,DEPARTIMENTO CHIM,I-40126 BOLOGNA,ITALY

[3] IST RIZZOLI,CNR,IST CITOMORFOL,BOLOGNA,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1052卷 / 02期

关键词：

(Erythroleukemia cell); Ornithine decarboxylase; Protein phosphorylation;

D O I：

10.1016/0167-4889(90)90232-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

32P-labeled ornithine decarboxylase was isolated by immunoprecipitation from murine erythroleukemia cells incubated in a medium containing [32P]ortophosphoric acid. Analysis of immunoprecipitate by SDS-polyacrylamide gel electrophoresis and autoradiography revealed a radiolabeled band, which corresponded to the position of mouse ornithine decarboxylase, phosphorylated in vitro by casein kinase-2. A preparation of casein kinase-2 purified from nuclei of erythroleukemia cells could also phosphorylate mouse ornithine decarboxylase. © 1990.

引用

页码：345 / 347

页数：3

共 17 条

[1] SYNTHESIS OF POLYPHOSPHOINOSITIDES IN NUCLEI OF FRIEND-CELLS - EVIDENCE FOR POLYPHOSPHOINOSITIDE METABOLISM INSIDE THE NUCLEUS WHICH CHANGES WITH CELL-DIFFERENTIATION [J].