LATEX PARTICLE AGGLUTINATION-TEST IN THE DIAGNOSIS OF HAEMOPHILUS-INFLUENZAE TYPE-B, STREPTOCOCCUS-PNEUMONIAE AND NEISSERIA-MENINGITIDIS-A AND NEISSERIA-MENINGITIDIS-C MENINGITIS IN INFANTS AND CHILDREN

被引:13
作者
CAMARGOS, PAM
ALMEIDA, MS
CARDOSO, I
FILHO, GL
FILHO, DM
MARTINS, JI
BATISTA, KWB
SILVA, RCO
ANTUNES, CMF
机构
[1] UNIV FED MINAS GERAIS, SCH MED & PARASITOL, INST BIOL SCI, DEPT PEDIAT, BELO HORIZONTE, MG, BRAZIL
[2] EZEQUIEL DIAS FDN, DIV MED BIOL, BELO HORIZONTE, MG, BRAZIL
[3] UNIV FED MINAS GERAIS, SCH MED, BELO HORIZONTE, MG, BRAZIL
关键词
PURULENT MENINGITIS; DIAGNOSIS LATEX PARTICLE AGGLUTINATION (FIXATION) TEST;
D O I
10.1016/0895-4356(95)00016-W
中图分类号
R19 [保健组织与事业(卫生事业管理)];
学科分类号
摘要
The knowledge of purulent meningitis etiology is essential in deciding the immediate therapy; in developing countries, however, the etiological agent identification does not reach 60% of the cases. A comparative study using the latex particle agglutination test (LPAT) in cerebrospinal fluid (CSF) for the diagnosis of meningitis due to Haemophilus influenzae type b, Streptococcus pneumoniae or Neisseria meningitidis A and C was carried out in Belo Horizonte MG, Brazil. CSF culture was used as a gold-standard. Two hundred and ninety-nine children, ranging from 3 months to 14 years of age, were included in the investigation. One hundred and forty-four presented a positive CSF culture for the above mentioned bacteria; the remaining presented meningitis due to other organisms (other bacteria or viral) or a normal CSF. The sensitivity and the specificity of LPAT was 95.7 and 100.0% for N. meningitidis C, 95.2 and 100.0% for H. influenzae type b and 86.5 and 100.0% for S. pneumoniae, respectively. When all three organisms were considered simultaneously, the sensitivity and the specificity was 93.0 and 100.0%, respectively. Taking into consideration a realistic estimate of disease prevalence in the community where the diagnostic test is being used, the positive predictive value and the posttest probability were estimated as 36.7 and 47.1% for children < 5 years and as 21.3 and 35.1% for children < 14 years of age, respectively. LPAT is a useful diagnostic test for meningitis due to the studied pathogens, especially in developing countries where laboratory facilities are limited.
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页码:1245 / 1250
页数:6
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