A1 ATP AS AN INTRACELLULAR CARRIER OF A1(III) ION

1. Using Al-27 and P-31 NMR spectroscopy in conjunction with an Al lactate aqueous reagent at pH 7.2, Al complexes of ATP and of phospholipids were characterized in synthetic-aqueous and organic-phospholipid chemical systems and in the intact human red blood cell. 2. The observed P-31 NMR chemical shifts of the Al-ATP complex in aqueous laboratory preparations or the intact human red blood cell were, respectively, alpha-phosphate, -11.53-delta; beta-phosphate, -22.65-delta; and gamma-phosphate, -10.95-delta. 3. The observed complexed Al-27 chemical shift was -2.22-delta. 4. The relative affinities for Al of the phospholipids determined from P-31 NMR spectroscopic titrations were PA >> Cl >> PS > PG congruent-to PI > PE plas congruent-to PE >> SPH > PC.