FUNCTIONAL-ROLE OF TTF-1 BINDING-SITES IN BOVINE THYROGLOBULIN PROMOTER

被引：29

作者：

JAVAUX, F

BERTAUX, F

DONDA, A

FRANCISLANG, H

VASSART, G

DILAURO, R

CHRISTOPHE, D

机构：

[1] UNIV LIBRE BRUXELLES,IRIBHN,CAMPUS ERASME,ROUTE LENN 808,B-1070 BRUSSELS,BELGIUM

[2] UNIV LIBRE BRUXELLES,DEPT GENET,B-1070 BRUSSELS,BELGIUM

[3] EUROPEAN MOLEC BIOL LAB,W-6900 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1992年 / 300卷 / 03期

关键词：

TRANSCRIPTION FACTOR; TTF-1; TG GENE;

D O I：

10.1016/0014-5793(92)80850-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the binding of purified TTF-1 on the bovine thyroglobulin gene promoter. DNase I footprinting experiments revealed three binding sites which corresponded in location to the A, B and C sites found in the rat thyroglobulin promoter. Mutants in the A and C regions showing reduced binding of TTF-1, also exhibited largely decreased promoter activity in transient expression experiments in primary-cultured dog thyrocytes. Two mutants in the B site that exhibited a reduced capacity to bind TTF-1 also displayed a drastically affected transcriptional activity in transient assays. As in the rat, sites A and C only are critical for promoter activity, these results suggest that full occupancy of the B site is required for thyroglobulin promoter activity in the cow only.

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页码：222 / 226

页数：5

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