EXPRESSION OF A MULTIFUNCTIONAL DNA-REPAIR ENZYME GENE, APURINIC APYRIMIDINIC ENDONUCLEASE (APE-REF-1) IN THE SUPRACHIASMATIC, SUPRAOPTIC AND PARAVENTRICULAR NUCLEI

被引:31
作者
RIVKEES, SA [1 ]
KELLEY, MR [1 ]
机构
[1] INDIANA UNIV,SCH MED,DEPT BIOCHEM & MOLEC BIOL,INDIANAPOLIS,IN 46202
关键词
SUPRACHIASMATIC NUCLEUS; SUPRAOPTIC NUCLEUS; CIRCADIAN RHYTHM; DNA REPAIR; FOS; APURINIC/APYRIMIDINIC ENDONUCLEASE;
D O I
10.1016/0006-8993(94)90296-8
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Apurinic/apyrimidinic endonuclease (APE; also referred to as Ref-l) repairs oxidative damage to DNA and regulates the redox state of DNA binding proteins. This later property influences the ability of DNA binding proteins, which include Fos and Jun, to bind to AP-1 complexes. Since DNA binding proteins may play important roles in regulating neuronal activity in the hypothalamus, we examined the expression of APE in the hypothalami of rats. In situ hybridization studies revealed high levels of APE mRNA expression in the suprachiasmatic nuclei (SCN), supraoptic nuclei (SON) and paraventricular nuclei (PVN). Since the SCN are the site of a biological clock, we examined whether APE gene expression was regulated by the circadian cycle or by light. Quantitative in situ hybridization studies showed that APE mRNA levels remained constant over the circadian cycle and were not increased by light exposure at night. We also tested if APE expression was under osmotic control in the SON and PVN. Hypertonic stimulus, however, did not induce further expression of APE mRNA in either the SON or the PVN. These findings identify the SCN, SON and PVN as sites of high level APE gene expression. These data suggest that APE may play an important role in these structures either to facilitate DNA repair or DNA binding protein action.
引用
收藏
页码:137 / 142
页数:6
相关论文
共 27 条
[1]   LIGHT REGULATES EXPRESSION OF A FOS-RELATED PROTEIN IN RAT SUPRACHIASMATIC NUCLEI [J].
ARONIN, N ;
SAGAR, SM ;
SHARP, FR ;
SCHWARTZ, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (15) :5959-5962
[2]   CLONING AND EXPRESSION OF APE, THE CDNA-ENCODING THE MAJOR HUMAN APURINIC ENDONUCLEASE - DEFINITION OF A FAMILY OF DNA-REPAIR ENZYMES [J].
DEMPLE, B ;
HERMAN, T ;
CHEN, DS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11450-11454
[3]   REDOX REDUX - THE CONTROL OF OXIDATIVE STRESS RESPONSES [J].
DEMPLE, B ;
AMABILECUEVAS, CF .
CELL, 1991, 67 (05) :837-839
[4]   THE ENZYMOLOGY OF APURINIC APYRIMIDINIC ENDONUCLEASES [J].
DOETSCH, PW ;
CUNNINGHAM, RP .
MUTATION RESEARCH, 1990, 236 (2-3) :173-201
[5]   C-FOS AND RELATED IMMEDIATE-EARLY GENE-PRODUCTS AS MARKERS OF ACTIVITY IN NEUROENDOCRINE SYSTEMS [J].
HOFFMAN, GE ;
SMITH, MS ;
VERBALIS, JG .
FRONTIERS IN NEUROENDOCRINOLOGY, 1993, 14 (03) :173-213
[6]   REGULATION OF JUN-B MESSENGER-RNA AND AP-1 ACTIVITY BY LIGHT AND A CIRCADIAN CLOCK [J].
KORNHAUSER, JM ;
NELSON, DE ;
MAYO, KE ;
TAKAHASHI, JS .
SCIENCE, 1992, 255 (5051) :1581-1584
[7]   PHOTIC AND CIRCADIAN REGULATION OF C-FOS-GENE EXPRESSION IN THE HAMSTER SUPRACHIASMATIC NUCLEUS [J].
KORNHAUSER, JM ;
NELSON, DE ;
MAYO, KE ;
TAKAHASHI, JS .
NEURON, 1990, 5 (02) :127-134
[8]   REPAIR OF INTRINSIC DNA LESIONS [J].
LINDAHL, T .
MUTATION RESEARCH, 1990, 238 (03) :305-311
[9]  
MOORE RY, 1983, FED PROC, V42, P2783
[10]  
MOORE RY, 1989, DEV CIRCADIAN RHYTHM, P1