A SIMPLE METHOD OF DNA EXTRACTION FROM WHOLE TISSUES AND BLOOD USING GLASS POWDER FOR DETECTION OF TRANSGENIC ANIMALS BY PCR

被引：21

作者：

ATTAL, J ^{[1
]}

CAJEROJUAREZ, M ^{[1
]}

HOUDEBINE, LM ^{[1
]}

机构：

[1] INRA,UNITE DIFFERENCIAT CELLULAIRE,F-78352 JOUY EN JOSAS,FRANCE

来源：

TRANSGENIC RESEARCH | 1995年 / 4卷 / 02期

关键词：

DNA EXTRACTION; GLASS POWDER; PCR; TRANSGENE;

D O I：

10.1007/BF01969417

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A very simple and reliable method to extract DNA directly from mouse tail, rabbit ear and blood is described. Tissue was homogenized in a solution of NaI and the DNA was extracted using glass powder. The extracted DNA was obtained in sufficient quantity and purity to allow direct detection of transgene by PCR.

引用

页码：149 / 150

页数：2

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